LncRNA ENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR-146b/AGPAT2 pathway.

The liver contributes to lipid metabolism as the hub of fat synthesis. Long non-coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes' lipid metabolism. Thirty-two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR-146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)-146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high-fat diet modulated the expression of lncRNA 21 686 and miR-146b (p-value < 0.001). Moreover, there was a significant increase in 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat-related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p-value < 0.05). Overexpression of miR-146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR-146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR-146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.

Adaptive Optimization Method for Prediction and Compensation of Thin-Walled Parts Machining Deformation Based on On-Machine Measurement.

Thin-walled aluminum alloy parts are widely used in the aerospace field because of their favorable characteristics that cater to various applications. However, they are easily deformed during milling, leading to a low pass rate of workpieces. On the basis of on-machine measurement (OMM) and surrogate stiffness models (SSMs), we developed an iterative optimization compensation method in this study to overcome the machining deformation of thin-walled parts. In the error compensation process, the time-varying factors of workpiece stiffness and the impact of prediction model errors were considered. First, we performed machining deformation simulation and information extraction on the key nodes of the machined surface, and an SSM containing the stiffness information of discrete nodes of each cutting layer was established. Subsequently, the machining errors were monitored through intermittent OMM to suppress the adverse impact of prediction model errors. Further, an interlayer correction coefficient was introduced in the compensation process to iteratively correct the prediction model error of each node in the SSM along the depth direction, and a correction coefficient between parts was introduced to realize the iterative correction of the prediction model for the same node position between different parts. Finally, the feasibility of the proposed method was verified through multiple sets of actual machining experiments on thin-walled parts with added pads.

AgMAsS3 (M = Cd, Hg): Double d10 Cation-Containing Thioarsenites(III) Exhibiting High Photocurrent Response and Moderate Second Harmonic Generation.

Thioarsenites(III) are an advanced functional material platform owing to the stereochemically active lone pair cations. In this paper, two novel quaternary thioarsenites(III), AgMAsS3 (M = Cd, Hg), are successfully obtained by introducing double d10 cations. In the compounds, d10 cations show a variety of different coordination modes ([AgS4] and [HgS4] in AgHgAsS3 vs [AgS5] and [CdS6] in AgCdAsS3). As a result, AgHgAsS3 and AgCdAsS3 crystallize in the noncentrosymmetric Cc space group and centrosymmetric C2/c space group, respectively. The band gaps of AgHgAsS3 and AgCdAsS3 are determined experimentally as 1.90 and 2.20 eV, respectively. Meanwhile, title compounds exhibit strong photocurrent responses. Specifically, AgHgAsS3 has a large birefringence of 0.18 at 2100 nm and a moderate second harmonic generation of (0.5 × AgGaS2). Moreover, the origin of linear and nonlinear optical responses is investigated based on first-principles calculations. This study enriches the family of MI-MII-As-Q (M = Ag, Cu; MII = Zn, Cd, Hg; Q = chalcogen) chalcogenides and helps to understand and design other multifunctional optical materials.

SrAgAsS4: A Noncentrosymmetric Sulfide with Good Infrared Nonlinear Optical Performance Induced by Aliovalent Substitution from Centrosymmetric SrGa2S4.

A new noncentrosymmetric (NCS) quaternary sulfide, SrAgAsS4, was obtained via the strategy of aliovalent substitution based on centrosymmetric (CS) SrGa2S4. The new compound features two-dimensional [AgAsS4]2- layers, which are composed of alternately connected [AsS4] tetrahedra and [AgS4] tetrahedra. Importantly, SrAgAsS4 exhibits a strong phase-matched second-harmonic generation response (1.35 × AgGaS2 at 2100 nm) and has a suitable birefringence (0.15@2100 nm) and moderate band gap (2.31 eV). The first-principles calculations revealed the significant contribution of [AsS4] and [AgS4] tetrahedra to its optical properties. This work will promote the application of the aliovalent substitution strategy in the design of NCS-structure-based functional materials.

Synthesis and Characterizations of Two Tellurides ß-BaGa2Te4 and Ba5Ga2Ge3Te12 with Flexible Chain Structure.

Demands for IR birefringent materials are increasing due to the rapid developments of IR laser applications. Herein, two new chain tellurides ß-BaGa2Te4 and Ba5Ga2Ge3Te12 have been discovered. ß-BaGa2Te4 crystallizes in the orthorhombic space group Imma (no. 74) with unit cell constants of a = 23.813(3) Å, b = 11.9673(19) Å, and c = 6.7215(9) Å, while Ba5Ga2Ge3Te12 crystallizes in the monoclinic space group P21/c (no. 14) with unit cell constants of a = 13.6540(3) Å, b = 9.6705(2) Å, and c = 23.1134(7) Å. The structure of ß-BaGa2Te4 can be considered to be the antiparallel arrangement of one-dimensional (1D) [GaTe2] chains formed by edge-sharing GaTe4 tetrahedra, which are separated by Ba2+ cations. In the crystal structure of Ba5Ga2Ge3Te12, two kinds of 1D chains, namely chain 1 ∞1[(GaGe)3Te8] and chain 2 ∞1[(GaGe)2Te4], are stacked alternately and put together by the coulomb force with Ba2+ cations. In addition, First-principles calculations indicate that ß-BaGa2Te4 has a large birefringence, ∼0.325 at 2050 nm, derived from the superposition of the polarizabilities of GaTe4 tetrahedra, implying that it has potential as an IR birefringent material. This work may provide some guidance for exploring new IR birefringent crystals.

Investigation into Structural Variation from 3D to 1D and Strong Second Harmonic Generation of the AHgPS4 (A+ = Na+, K+, Rb+, Cs+) Family.

The continuous exploration of multinary chalcogenide semiconductors has provided a variety of new functional materials. In this paper, four new quaternary chalcogenides AHgPS4 (A+ = Na+, K+, Rb+, Cs+) have been prepared by solid-state syntheses. These findings complement the lack of research on this quaternary system. Influenced by the size effect of cations and the coordination mode of Hg, the four compounds crystallize in four different space groups [NaHgPS4, P4̅n2; KHgPS4, Pnn2; RbHgPS4, P21/n; CsHgPS4, P212121] and show an interesting evolution from a 3D framework structure to a 1D chain structure. Moreover, all of these compounds feature noncentrosymmetric (NCS) structures except for RbHgPS4. The materials exhibit wide band gaps of 2.7 eV < Eg < 3.0 eV. The NCS- related second-harmonic-generation (SHG) property of NaHgPS4 and KHgPS4 was also studied. They display strong powder SHG responses (3.14 × AgGaS2 for NaHgPS4; 4.15 × AgGaS2 for KHgPS4), which indicate their intriguing potential as IR nonlinear-optical materials. Moreover, first-principles theoretical calculations were performed to understand the structure-property relationships of these materials.

Na3Bi(IO3)6: An Alkali-Metal Bismuth Iodate with Intriguing One-Dimensional [BiI6O18] Chains and Pressure-Induced Structural Transition.

An alkali-metal bismuth iodate, Na3Bi(IO3)6, was successfully obtained by the hydrothermal method for the first time and contains intriguing one-dimensional [BiI6O18] chains. High-pressure Raman spectra were carried out to investigate the structural transition of Na3Bi(IO3)6. Electronic states and anisotropic optical responses were also investigated by theoretical calculations.

Two Mixed-Anion Units of [GeOSe3] and [GeO3S] Originating from Partial Isovalent Anion Substitution and Inducing Moderate Second Harmonic Generation Response and Large Birefringence.

Highly polarizable mixed-anion structural building units (SBUs) have been demonstrated as promising candidates for high-performing optical crystals. In this work, two new mixed-anion SBUs of [GeOSe3] and [GeO3S] are first designed through partial isovalent substitution of chalcogen atoms by O atoms in the classical [GeQ4] (Q = S, Se) tetrahedra. On the basis of these SBUs, two new quaternary oxychalcogenides, Sr3Ge2O4Se3 and SrGe2O3S2, are successfully synthesized. Sr3Ge2O4Se3 crystallizes in the noncentrosymmetric space group R3m and possesses unique zero-dimensional [Ge2O4Se3]6- units consisting of highly distorted [GeOSe3] tetrahedra and [GeO4] tetrahedra through a shared O atom. It displays intriguing potential as an infrared nonlinear optical material with a wide band gap (2.96 eV) and moderate second harmonic generation intensity (0.8 × AgGaS2). SrGe2O3S2 belongs to the centrosymmetric space group P21/c and features 2∞[Ge2O3S2]2- layers formed by the corner-shared [GeO3S] tetrahedra. Moreover, the large birefringence of SrGe2O3S2 (calculated Δn = 0.22-0.17 from 0.4 to 4.0 µm) gives it a potential as a birefringent material. Theoretical calculations revealed the crucial effects of mixed-anion [GeOSe3] and [GeO3S] units on the moderate second harmonic generation response and large birefringence. The discovery of new mixed-anion SBUs of [GeOSe3] and [GeO3S] will guide the exploration of new functional oxychalcogenides.

Functional Chalcogenide Na2HgSn2Se6 and K2MnGe2Se6 Exhibiting Flexible Chain Structure and Intriguing Birefringence Tunability.

The two functional chalcogenides K2MnGe2Se6 and Na2HgSn2Se6, featuring a straight-chain structure, have been successfully prepared and fully characterized. K2MnGe2Se6 shows paramagnetic behavior. The birefringence of Na2HgSn2Se6 is as large as 0.3107 and derives from the superposition of the polarizabilities of its fundamental building blocks, on the basis of first-principles calculations. Moreover, the flexible framework of the A2MIIMIV2Se6 family enables a variety of heterogeneous substitutions and thus offers possible birefringence tunability, which may inspire the design and exploratory synthesis of IR birefringent materials.

EuHgGeSe4 and EuHgSnS4: Two Quaternary Eu-Based Infrared Nonlinear Optical Materials with Strong Second-Harmonic-Generation Responses.

Metal chalcogenides play a critical role in the infrared (IR) nonlinear optical (NLO) field. However, Eu-based chalcogenide-type IR NLO materials are still scarce up to now. In this paper, two new quaternary Eu-based chalcogenides, EuHgGeSe4 and EuHgSnS4, containing the "NLO active groups" [HgQ4]6- (Q = S, Se) and [GeSe4]4-/[SnS4]4- were synthesized through traditional high-temperature solid-state reactions. They possess noncentrosymmetric structures, crystallizing in the Ama2 space group, and exhibit strong phase-matchable second-harmonic-generation (SHG) responses (3.1× and 1.77× that of AgGaS2 for EuHgGeSe4 and EuHgSnS4, respectively). Meanwhile, the optical band gaps of EuHgGeSe4 (1.97 eV) and EuHgSnS4 (2.14 eV) were determined from UV-vis-NIR diffuse reflectance spectra. Differential scanning calorimetry (DSC) analyses reveal the congruent-melting behavior of EuHgGeSe4. Furthermore, structural analysis and theoretical calculations verify the critical driving effects of [HgQ4]6- tetrahedra on the strong SHG activity. The overall results demonstrate that EuHgGeSe4 and EuHgSnS4 are potential IR NLO materials.

AHgSnQ4 (A = Sr, Ba; Q = S, Se): A Series of Hg-Based Infrared Nonlinear-Optical Materials with Strong Second-Harmonic-Generation Response and Good Phase Matchability.

Four Hg-based IR nonlinear-optical materials, AHgSnQ4 (A = Sr, Ba; Q = S, Se), were discovered and investigated systematically. Their structures are built of two-dimensional [HgSnQ4]2- layers, which are assembled alternately by distorted (HgQ4 and SnQ4) tetrahedra and separated by eight-coordinated A2+ cations. The two sulfides AHgSnS4 (A = Ba, Sr) exhibit large second-harmonic-generation (SHG) responses (2.8 and 1.9 × AgGaS2 at 2.09 µm), as well as large band gaps (2.77 and 2.72 eV). The two selenides AHgSnSe4 (A = Ba, Sr) show even stronger SHG responses, about 5 times that of AgGaS2. Furthermore, all four compounds show phase-matching behavior, and the results of first-principles calculation elucidate the key role of the HgQ4 group in the enhanced SHG effect in ß-BaHgSnS4 and BaHgSnSe4.

Ultrastable 3D cage-like metal-organic frameworks constructed using polydentate cyanurate ligands and their gas adsorption properties.

Stability is a key factor that restricts the practical applications of metal-organic framework (MOF) materials. In this work, we report an ultrastable three-dimensional cage-like MOF, SrCu(HC3N3O3)2, constructed by a polydentate cyanurate ligand and two kinds of different metal nodes. A high ratio of coordination sites in organic ligands, specific coordination of strong acid with a strong base and weak acid with a weak base and double independent completed coordination networks endow SrCu(HC3N3O3)2 with outstanding thermal stability (up to 300 °C) and acid/alkali resistance (pH = 2-14). Moreover, SrCu(HC3N3O3)2 possesses the highest porosity up to 36.7% among cyanuric acid-based MOF materials and exhibits differentiated adsorption of C3H4 (63 cm3 g-1) and C3H6 (51 cm3 g-1). The breakthrough experiment further verified that efficient C3H4/C3H6 separation can be achieved under dynamic conditions by SrCu(HC3N3O3)2.

[Ba4X][In19S32] (X = Cl, Br): two quaternary metal chalcohalides exhibiting remarkable photocurrent responses.

Inorganic metal chalcohalides, as significant semiconductor materials, have emerged as promising candidates for photoelectric applications. Herein, a new type of quaternary chalcohalide, [Ba4X][In19S32] (X = Cl, Br), has been discovered using the high-temperature halide salt flux method. Single-crystal X-ray diffraction analysis reveals that they are isostructural and crystallize in the tetragonal space group I41/amd (no. 141) featuring the octahedral hole formed by six [InS4]5- tetrahedra filled with a [ClBa4]7+ polycation, surrounded by a three-dimensional covalent framework formed by interconnecting [InS6]9- octahedra through corner-sharing and edge-sharing. Moreover, [Ba4Cl][In19S32] and [Ba4Br][In19S32] exhibit wide optical bandgaps of 2.70 eV and 2.46 eV, respectively, and moderate birefringences (0.044 @ 2100 nm and 0.042 @ 2100 nm, respectively). Specifically, [Ba4X][In19S32] (X = Cl, Br) display remarkable photocurrent responses under simulated solar-light illumination, implying their potential for photocatalytic applications. Theoretical calculations were employed to understand the interrelationship between the optical properties and electronic structure. The study on the synthesis and structure-property relationship analysis of inorganic metal chalcohalides provides new insight into the exploration of promising photoelectric materials.

Quaternary alkaline-earth metal thiophosphate SrAgPS4: syntheses, structures, and optical properties.

Metal thiophosphates have outstanding properties for the generation of mid-infrared coherent light and are an emerging nonlinear optical material system. In this study, a new non-centrosymmetric (NCS) quaternary alkaline-earth metal thiophosphate, SrAgPS4, was obtained via a high-temperature solid-state method. The new compound crystallizes in the NCS Ama2 (No. 40) space group and features two-dimensional [AgPS4]2- layers consisting of alternately connected [PS4] and [AgS4] tetrahedra. SrAgPS4 exhibits a strong phase-matched second harmonic generation response (1.10 × AgGaS2 at 2100 nm) and a large band gap (2.97 eV). In addition, theoretical calculations reveal the intrinsic relationship between the electronic structure and optical properties. This work enriches and greatly promotes the research on infrared nonlinear optical materials based on thiophosphates.

Bridging oxygen atoms in trigonal prism units driven strong second-harmonic-generation efficiency in Sr3Ge2O4Te3.

Herein, a novel IR NLO oxytelluride Sr3Ge2O4Te3 was successfully designed and synthesized through a "partial O-to-Te substitution" strategy. Compared with the parent oxide, Sr3Ge2O4Te3 not only successfully achieves a phase-matchability transition (from NPM to PM), but also greatly improves the linear and NLO performances, including a wide band gap (2.26 eV), strong SHG response (1.3 × AgGaSe2) and large optical anisotropy (Δn = 0.152@2090 nm). The analyses of the structure-property relationship and SHG-density indicate that the bridging oxygen in the [O3Ge-O-GeTe3] prism unit plays the most important role in the multiplication SHG effect. This work provides some insights into the design and exploration of novel IR NLO materials.

The synthesis and structure-property relation analysis of metal chalcohalide crystals Cs2InPS4X2 (X = Cl, Br) with mixed anions.

Inorganic metal chalcohalides are significant semiconductive materials for photovoltaics, photodetetion and infrared optics. Thus it is considerably rewarding to develop a new synthetic strategy to provide more degrees of freedom for atomic coordination to tune the optical and electronic properties of metal chalcohalides. In this work, the mixed-anion strategy is performed to synthesize two new metal chalcohalides Cs2InPS4X2 (X = Cl, Br) with mixed-anion structure by the reaction of InPS4 and CsX. Single-crystal X-ray diffraction analysis shows that they are isostructural and crystallize in the centrosymmetric space group P21/n, consisting of zero-dimensional structure [In2P2S8X4]4- (X = Cl, Br) built from tetrahedral [PS4]3- and octahedral [InS4X2]7- (X = Cl, Br) through edge-sharing, with Cs cations filling in intervening voids. The UV-vis-NIR diffuse reflectance spectroscopy measurement reveals that Cs2InPS4Cl2 and Cs2InPS4Br2 exhibit large optical bandgaps of 3.21 eV and 3.12 eV, respectively. The electronic structure calculations show that the bandgap mainly originates from the [InS4X2]7- (X = Cl, Br) mixed-anion groups. First-principles calculations indicate that the birefringence of Cs2InPS4Cl2 and Cs2InPS4Br2 is ∼0.08 and ∼0.05 at 2090 nm, respectively. Furthermore, thermal analysis reveals that the Cs2InPS4X2 (X = Cl, Br) are thermostable up to 400 °C. This discovery enriches the structural diversity of inorganic chalcohalides and provides an insight for the exploration of new semiconductive materials.

Immune Response of Eastern Honeybee Worker to Nosema ceranae Infection Revealed by Transcriptomic Investigation.

Here, a comparative transcriptome investigation was conducted based on high-quality deep sequencing data from the midguts of Apis cerana cerana workers at 7 d post-inoculation (dpi) and 10 dpi with Nosema ceranae and corresponding un-inoculated midguts. PCR identification and microscopic observation of paraffin sections confirmed the effective infection of A. c. cerana worker by N. ceranae. In total, 1127 and 957 N. ceranae-responsive genes were identified in the infected midguts at 7 dpi and 10 dpi, respectively. RT-qPCR results validated the reliability of our transcriptome data. GO categorization indicated the differentially expressed genes (DEGs) were respectively engaged in 34 and 33 functional terms associated with biological processes, cellular components, and molecular functions. Additionally, KEGG pathway enrichment analysis showed that DEGs at 7 dpi and 10 dpi could be enriched in 231 and 226 pathways, respectively. Moreover, DEGs in workers' midguts at both 7 dpi and 10 dpi were involved in six cellular immune pathways such as autophagy and phagosome and three humoral immune pathways such as the Toll/Imd signaling pathway and Jak-STAT signaling pathway. In addition, one up-regulated gene (XM_017055397.1) was enriched in the NF-κB signaling pathway in the workers' midgut at 10 dpi. Further investigation suggested the majority of these DEGs were engaged in only one immune pathway, while a small number of DEGs were simultaneously involved in two immune pathways. These results together demonstrated that the overall gene expression profile in host midgut was altered by N. ceranae infection and some of the host immune pathways were induced to activation during fungal infection, whereas some others were suppressed via host-pathogen interaction. Our findings offer a basis for clarification of the mechanism underlying the immune response of A. c. cerana workers to N. ceranae infection, but also provide novel insights into eastern honeybee-microsporodian interaction.

Highly Distorted [HgS4] Motif-Driven Structural Symmetry Degradation and Strengthened Second-Harmonic Generation Response in the Defect Diamond-Like Chalcogenide Hg3P2S8.

Chalcogenides with diamond-like (DL) structures are a treasury of infrared nonlinear optical (NLO) materials. Here, a ternary Hg-based chalcogenide with a defect DL structure, Hg3P2S8, is synthesized by solid-state reaction. Driven by the highly distorted [HgS4] tetrahedra, this compound displays an interesting structural symmetry degradation from tetragonal to orthorhombic compared with its analogue Zn3P2S8. Meanwhile, the overall performances of Hg3P2S8 are quite remarkable, including a very strong phase-matchable second-harmonic generation (SHG) response (4.2 × AgGaS2), large band gap (2.77 eV), wide IR transparent range (0.45-16.7 µm), and high laser-induced damage threshold (4 × AGS). Furthermore, the theoretical analysis and local dipole moment calculations elucidate that the highly distorted [HgS4] tetrahedra contribute a lot to the enhancement of the SHG effect. This discovery will motivate the exploration of other DL Hg-based chalcogenides serving as high-performing mid-IR NLO materials.

Ba8SrPb24O24Cl18: the first alkali-earth metal lead(ii) oxyhalide with an intriguing multimember-ring layer.

The first alkali-earth metal lead(ii) oxyhalide Ba8SrPb24O24Cl18 characterized by fascinating multimember-ring layers has been discovered. Theoretical and experimental investigations illustrate that Ba8SrPb24O24Cl18 exhibits a moderate band gap of 3.09 eV, incongruent melting behavior and birefringence of 0.014@1064 nm. This discovery may offer new ideas for regulating the optical properties of oxyhalides and broadening their structural diversity.

New quaternary chalcogenide Ba4HgAs2S10 originating from the combination of linear [HgS2]2- and tetrahedral [AsS4]3- modules.

A new quaternary chalcogenide Ba4HgAs2S10 has been successfully synthesized with the aid of a KI flux. The compound crystallizes in the space group C2/c (no. 9) of the monoclinic system [a = 22.7787(6) Å, b = 6.4712(2) Å, c = 25.0606(7) Å, ß = 90.101(2)° and Z = 8]. It is the first example of tetrahedral [AsS4]3- and linear [HgS2]2- units coexisting in a single compound. The [AsS4]3- tetrahedra and [HgS2]2- units are totally separated by Ba2+. The UV-visible diffuse reflectance spectrum reveals a large bandgap of 2.98 eV for Ba4HgAs2S10 and DSC measurement demonstrates the incongruent melting nature of the compound. Moreover, based on first-principles calculations, Ba4HgAs2S10 is a direct bandgap semiconductor with the optical property related to the electron transition from the S-3p orbital to As-4p and Ba-5d orbitals.