Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion.

BACKGROUND: The incidence of gastric cancer ranks the first among digestive tract tumors in China. However, there are no specific symptoms in the early stage of the tumor and the diagnosis process is complex, so more effective detection methods are very needed. In this study, a novel long noncoding RNA (lncRNA) was introduced as a diagnostic biomarker for gastric cancer, which brought new thinking to the exploration of its pathological mechanism and clinical prediction. METHODS: The level of lncRNA EPB41L4A-AS1 (EPB41L4A-AS1) in gastric cancer serum and cells was verified via real-time quantitative polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curve was performed based on the EPB41L4A-AS1 level, and the diagnostic possibility of EPB41L4A-AS was analyzed. The chi-square test evaluated the correlation between EPB41L4A-AS expression and clinical information. The cells were cultured and transfected in vitro, and the mediations of abnormal EPB41L4A-AS level on the viability and motility of gastric cancer cells were verified through cell counting kit-8 (CCK-8) and Transwell assay. Furthermore, luciferase activity assay was performed to confirm the sponge molecule microRNA-17-5p (miR-17-5p) of EPB41L4A-AS1. RESULTS: EPB41L4A-AS1 was decreased in gastric cancer, and low EPB41L4A-AS1 level indicated resultful diagnostic value. Overexpression of EPB41L4A-AS1 inhibited the activity of gastric cancer cells, while knockdown of EPB41L4A-AS1 promoted tumor deterioration. EPB41L4A-AS1 directly targeted and regulated the expression ofmiR-17-5p. CONCLUSION: This study elaborated that EPB41L4A-AS1 is lowly expressed in gastric cancer. Silencing EPB41L4A-AS1 was beneficial to cell proliferation, migration, and invasion. EPB41L4A-AS1 provides a new possibility for the diagnosis of gastric cancer patients by targeting miR-17-5p.

SBP1 promotes tumorigenesis of thyroid cancer through TXN/NIS pathway.

BACKGROUND: As the tissue with the highest selenium content in the body, the occurrence and development of thyroid cancer are closely related to selenium and selenoproteins. Selenium-binding protein 1 (SBP1) has been repeatedly implicated in several cancers, but its role and molecular mechanisms in thyroid cancer remains largely undefined. METHODS: The expression of SBP1, sodium/iodide symporter (NIS) and thioredoxin (TXN) were analyzed in clinical samples and cell lines. Cell counting kit-8 (CCK-8) and tube formation assays were used to analyze the cell viability and tube formation of cells. Immunofluorescence was used to determine the expression of the NIS. Co-immunoprecipitation (Co-IP) assay was carried out to verify the interaction of SBP1 with TXN. The mouse xenograft experiment was performed to investigate the growth of thyroid cancer cells with SBP1 knockdown in vivo. RESULTS: SBP1 was significantly increased in human thyroid cancer tissues and cells, especially in anaplastic thyroid cancer. Overexpression of SBP1 promoted FTC-133 cell proliferation, and the culture supernatant of SBP1-overexpression FTC-133 cells promoted tube formation of human retinal microvascular endothelial cells. Knockdown of SBP1, however, inhibited cell proliferation and tube formation. Furthermore, overexpression of SBP1 inhibited cellular differentiation of differentiated thyroid cancer cell line FTC-133, as indicated by decreased expression of thyroid stimulating hormone receptors, thyroglobulin and NIS. Knockdown of SBP1, however, promoted differentiation of BHT101 cells, an anaplastic thyroid cancer cell line. Notably, TXN, a negative regulator of NIS, was found to be significantly upregulated in human thyroid cancer tissues, and it was positively regulated by SBP1. Co-IP assay implied a direct interaction of SBP1 with TXN. Additionally, TXN overexpression reversed the effect of SBP1 knockdown on BHT101 cell viability, tube formation and cell differentiation. An in vivo study found that knockdown of SBP1 promoted the expression of thyroid stimulating hormone receptors, thyroglobulin and NIS, as well as inhibited the growth and progression of thyroid cancer tumors. CONCLUSION: SBP1 promoted tumorigenesis and dedifferentiation of thyroid cancer through positively regulating TXN.

Laser Electrochemical Deposition Hybrid Preparation of an Oil-Water Separation Mesh with Controllable Pore Diameter Based on a BP Neural Network.

With the increasing problem of water pollution, oil-water separation technology has attracted widespread attention worldwide. In this study, we proposed laser electrochemical deposition hybrid preparation of an oil-water separation mesh and introduced a back-propagation (BP) neural network model to realize the regulation of metal filter mesh. Among them, the coating coverage and electrochemical deposition quality were improved by laser electrochemical deposition composite processing. Based on the BP neural network model, the pore size after electrochemical deposition could be obtained only by inputting the processing parameters into the model, enabling the prediction and control of the pore size of the processed stainless-steel mesh (SSM), and the maximum residual difference between the predicted value and the experimental value was 1.5%. According to the oil-water separation theory and practical requirements, the corresponding electrochemical deposition potential and electrochemical deposition time were determined by the BP neural network model, which reduced the cost and time loss. In addition, the prepared SSM was found to achieve efficient separation of oil and water mixtures, reaching 99.9% separation efficiency in a combination with oil-water separation, along with other performance tests without chemical modification. The prepared SSM showed good mechanical durability and the separation efficiency exceeded 95% after sandpaper abrasion, thus, still maintaining the separation ability of oil-water mixture. Compared to other similar preparation methods, the method proposed in this study has the advantages of controllable pore size, simplicity, convenience, environmental friendliness, and durable wear resistance, offering important application potential in the treatment of oily wastewater.

FOXE1 supports the tumor promotion of Gli2 on papillary thyroid carcinoma by the Wnt/ß-catenin pathway.

Papillary thyroid carcinoma (PTC) is a common malignancy in thyroid tissue. However, the molecular mechanism of PTC tumor progression remains unknown. The hedgehog (Hh) pathway is thought to play a key role during PTC development. Here we investigate the effects of glioma-associated oncogene protein-2 (Gli2), an important transcription factor of the Hh-signaling pathway, on PTC. Gli2 and forkhead box E1 (FOXE1) protein levels were upregulated in tissues of PTC patients and PTC cell lines. Using the PTC cell line TPC-1, we show that Gli2 small interfering RNA (siRNA) reduces cell proliferation, migration, and invasion; whereas overexpression of FOXE1 produces the opposite effects. Moreover, Gli2 siRNA inhibited the expression of genes implicated in the Wnt/ß-catenin pathway and that FOXE1 overexpression produces the opposite effects. Thus, it was indicated that Gli2 promoted the proliferation, migration, and invasion of TPC-1 cells by activating Wnt/ß-catenin and FOXE1 is involved in this process. Xenograft models of PTC were also constructed, the results showed that Gli2 siRNA reduced the rate of tumor growth, FOXE1 levels, and the expression of the Wnt/ß-catenin pathway but FOXE1 overexpression reversed that effects. In conclusion, this study demonstrates that Gli2 promotes the growth of PTC tumors and TPC-1 cell proliferation, migration, and invasion by activating the Wnt/ß-catenin pathway via FOXE1.

Nanosecond Laser-Induced Underwater Superoleophobic and Underoil Superhydrophobic Mesh for Oil/Water Separation.

Materials with special wettability have drawn considerable attention especially in the practical application for the separation and recovery of the oily wastewater, whereas there still remain challenges of the high-cost materials, significant time, and complicated production equipment. Here, a simple method to fabricate the underwater superoleophobic and underoil superhydrophobic brass mesh via the nanosecond laser ablation is reported for the first time, which provided the micro-/nanoscale hierarchical structures. This mesh is superhydrophilic and superoleophilic in air but superoleophobic under water and superhydrophobic under oil. On the basis of the special wettability of the as-fabricated mesh, we demonstrate a proof of the light or heavy oil/water separation, and the excellent separation efficiencies (>96%) and the superior water/oil breakthrough pressure coupled with the high water/oil flux are achieved. Moreover, the nanosecond laser technique is simple and economical, and it is advisable for the large-area and mass fabrication of the underwater superoleophobic and underoil superhydrophobic mesh in the large-scale oil/water separation.

Tenascin-C promotes migration of hepatic stellate cells and production of type I collagen.

Tenascin-C (TN-C) is an extracellular matrix glycoprotein markedly upregulated during liver fibrosis. The study is performed to explore the role of TN-C during the growth and activation of hepatic stellate cells (HSCs). We found that TN-C was accumulated accompanying with the HSC activation. Our data on cell migration assay revealed that the rTN-C treatment enhanced HSC migration in a dose- and time-dependent manner, but did not influence their proliferation. HSCs transfected with pTARGET-TN-C overexpression vector displayed increased the type I collagen (Col I) production. TN-C overexpression enhanced the process of HSC activation through TGF-ß1 signaling. Moreover, the anti-α9ß1 integrin antibody treatment blocked the TN-C-driven Col I increase in rat HSCs. Collectively, TN-C had a positive role in activation of HSCs mediated by TGF-ß1 and α9ß1 integrin, manifesting elevation of Col I production and promotion of cell migration. Our results provide a potential insight for the therapy of hepatic fibrosis.

Musashi1 regulates survival of hepatoma cell lines by activation of Wnt signalling pathway.

BACKGROUND & AIMS: Musashi1 (MSI1) belongs to the RNA-binding protein (RBP) family, with functions as translational activator or suppressor of specifically bound mRNA. However, its function in hepatocellular carcinoma (HCC) has been deeply unexplored. Here, we investigated the role of MSI1 for proliferation and tumourigenesis in HCC. METHODS: The expression of MSI1 in HCC tissues was examined by immunohistochemistry and western blotting. The effects of MSI1 overexpression and silencing on cell proliferation, cell viability, tumoursphere and tumour formation of HCC were explored. RESULTS: In this study, we initially reported that MSI1 was upregulated in HCC. Overexpression of MSI1 in HepG2 cell lines resulted in significantly promoted cell growth, tumour formation and cell cycle progression. Consistently, knockdown of MSI1 in Huh7 cell lines remarkably inhibited cell growth and tumour formation, and caused cell cycle arrest at the G1/S transition. Dual-luciferase assays indicated that MSI1 activated Wnt signal pathway, and APC and DKK1 were direct targets of MSI1. CONCLUSION: Taken together, these findings indicate that an oncogenic role of MSI1 in HCC may be through modulation of cell growth and cell cycle by activating Wnt pathway via direct downregulation of APC and DKK1.

Polymorphisms of PRLHR and HSPA12A and risk of gastric and colorectal cancer in the Chinese Han population.

BACKGROUND: Gastric and colorectal cancers have a major impact on public health, and are the most common malignant tumors in China. The aim of this research was to study whether polymorphisms of CHCHD3P1-HSP90AB7P, GRID1, HSPA12A, PRLHR, SBF2, POLD3 and C11orf93-C11orf92 genes are associated with the risk of gastric and colorectal cancers in the Chinese Han population. METHODS: We genotyped seven single nucleotide polymorphisms (SNPs) from seven genes. We selected 588 patients with gastric cancer and 449 with colorectal cancer, along with 703 healthy controls. All these SNPs were evaluated using the χ² test and genetic model analysis. RESULTS: The genotype "A/T" of rs12413624 in PRLHR gene was associated with a decreased risk of colorectal cancer in allele model analysis [odds ratio (OR) = 0.81; 95% confidence interval (CI) = 0.68-0.97; p = 0.018] and log-additive model analysis (OR = 0.81; 95% CI = 0.66-0.98; p = 0.032). The genotype "A/G" of rs1665650 in HSPA12A gene was associated with a decreased risk of gastric cancer in overdominant model analysis (OR = 0.77; 95% CI = 0.60-0.99; p = 0.038). CONCLUSIONS: Our results provide evidence that variants of PRLHR gene are a protective factor in colorectal cancer and variants of HSPA12A gene are a protective factor in gastric cancer in the Chinese Han population.

The Roles of Microprobe in Localized Electrodeposition: Electrolyte Localized Transport and Force-Displacement Sensitivity.

Facing the rapid development of 6G communication, long-wave infrared metasurface and biomimetic microfluidics, the performance requirements for microsystems based on metal tiny structures are gradually increasing. As one of powerful methods for fabrication metal complex microstructures, localized electrochemical deposition microadditive manufacturing technology can fabricate copper metal micro overhanging structures without masks and supporting materials. In this study, the role of the microprobe cantilever (MC) in localized electrodeposition was studied. The MC can be used for precise deposition with electrolyte localized transport function and high accuracy force-displacement sensitivity. To prove this, the electrolyte flow was simulated when the MC was in bending or normal state. The simulation results can indicate the influence of turbulent flow on the electrolyte flow velocity and the pressure at the end of the pyramid. The results show that the internal flow velocity increased by 8.9% in the bending probe as compared with normal. Besides, this study analyzed the force-potential sensitivity characteristics of the MC. Using the deformation of the MC as an intermediate variable, the model of the probe tip displacement caused by the growth of the deposit and the voltage value displayed by the photodetector was mathematically established. In addition, the deposition of a single voxel was simulated by simulation process with the simulated height of 520 nm for one voxel, and the coincidence of simulation and experimental results was 93.1%. In conclusion, this method provides a new way for localized electrodeposition of complex microstructures.

Chromosome-scale genome assemblies of sexually dimorphic male and female Acrossocheilus fasciatus.

Acrossocheilus fasciatus is a stream-dwelling fish species of the Barbinae subfamily. It is valued for its colorfully striped appearance and delicious meat. This species is also characterized by apparent sexual dimorphism and toxic ovum. Biology and aquaculture researches of A. fasciatus are hindered by the lack of a high-quality reference genome. Here, we report chromosome-level genome assemblies of the male and female A. fasciatus. The HiFi-only genome assemblies for both female and male individuals were 899.13 Mb (N50 length of 32.58 Mb) and 885.68 Mb (N50 length of 33.06 Mb), respectively. Notably, a substantial proportion of the assembled sequences, accounting for 96.15% and 98.35% for female and male genomes, respectively, were successfully anchored onto 25 chromosomes utilizing Hi-C data. We annotated the female assembly as a reference genome and identified a total of 400.62 Mb (44.56%) repetitive sequences, 27,392 protein-coding genes, and 35,869 ncRNAs. The high-quality male and female reference genomes will provide genomic resources for developing sex-specific molecular markers, inform single-sex breeding, and elucidate genetic mechanisms of sexual dimorphism.

Laser Interference Additive Manufacturing: Mask Bundle Shape Bionic Shark Skin Structure.

Here, we explored a new manufacturing strategy that uses the mask laser interference additive manufacturing (MLIAM) technique, which combines the respective strengths of laser interference lithography and mask lithography to efficiently fabricate across-scales three-dimensional bionic shark skin structures with superhydrophobicity and adhesive reduction. The phenomena and mechanisms of the MLIAM curing process were revealed and analyzed, showing the feasibility and flexibility. In terms of structural performance, the adhesive force on the surface can be tuned based on the growth direction of the bionic shark skin structures, where the maximum rate of the adhesive reduction reaches about 65%. Furthermore, the evolution of the directional diffusion for the water droplet, which is based on the change of the contact angle, was clearly observed, and the mechanism was also discussed by the models. Moreover, no-loss transportations were achieved successfully using the gradient adhesive force and superhydrophobicity on the surface by tuning the growth direction and modifying by fluorinated silane. Finally, this work gives a strategy for fabricating across-scale structures on micro- and nanometers, which have potential application in bioengineering, diversional targeting, and condenser surface.

Fabrication of durable underoil superhydrophobic surfaces with self-cleaning and oil-water separation properties.

In this study, a simple method without any additional chemical modification is proposed to fabricate underoil superhydrophobic surfaces with micro- and nano-hierarchical structures using a nanosecond laser system. The fabricated surfaces exhibited extreme superhydrophobicity and underoil superhydrophobicity with high contact angles of 153.8 ± 1.5° and 161.3 ± 1.1°, respectively. The results show that even after 20 abrasion cycles, the fabricated surfaces retained water repellency and self-cleaning performance under oil, while the superhydrophobicity in air was not resistant to wear. In addition, the fabricated brass meshes can also be used to separate oil in an oil-water mixture based on the prewetting induced underoil superhydrophobicity after being damaged. The separation efficiency was as high as 97.8%, which made them more appropriate for the oil-water separation than those based on superhydrophobicity. The proposed fabrication method is suitable for large-scale and mass production and provides a new avenue and possibility for further development of robust functional interface materials.

Laser-Induced Slippery Liquid-Infused Surfaces with Anticorrosion and Wear Resistance Properties on Aluminum Alloy Substrates.

Slippery liquid-infused surfaces (SLISs) are developed as a potential alternative to superhydrophobic surfaces (SHSs) to resolve the issues of poor durability in corrosion protection and wear resistance. In this work, we used a simple laser processing technology to prepare a SLIS on the aluminum alloy (7075) surface. The superhydrophobicities of the modified surface and the oil film formed by liquid injection make the corrosive medium difficult to directly contact the surface and thus have a significant effect on corrosion resistance. The water and oil repellent SLIS exhibits durable corrosion resistance and excellent tribological properties compared with the SHS. The anticorrosion and wear resistance performances provided by the composite film have been assessed by multiple methods including the electrochemical test, immersion test, and friction wear test. The results indicate that compared to the bare surface, laser-ablated surface (LAS), and fluoroalkyl silane-modified SHS, the SLIS composite coating has better corrosion resistance and wear resistance, which is of great significance to expand the potential applications of 7075 aluminum alloys. The work provides a research basis for expanding the practical application of SLISs in complex environments.

Evaluation of Three-Dimensional Surface Roughness in Microgroove Based on Bidimensional Empirical Mode Decomposition.

Micromilling is an extremely important advanced manufacturing technology in the micromanufacturing industry. Compared with the traditional milling process, micromilling has stricter requirements on the surface roughness of the workpiece, and the roughness of the microcurved surface is not easy to measure. In order to more accurately characterize the curved surface morphology of the microgrooves obtained by micromilling, this paper proposes a method to extract the reference plane of the curved surface based on the bidimensional empirical mode decomposition algorithm and characterize the three-dimensional surface roughness of the curved surface. First, we synthesize the morphologies of the microgrooves by simulated non-Gaussian rough surfaces and models of textures. Second, the bidimensional empirical mode decomposition algorithm was used to extract the reference planes of the simulated microgrooves. Third, the three-dimensional roughness parameters suitable for the curved surfaces of microgrooves were selected to establish an evaluation system. The results show that the mean squared errors of the reference planes are below 1%, so bidimensional empirical mode decomposition can effectively extract reference planes, and the evaluation system of three-dimensional surface roughness proposed in this paper reflects morphological characteristics of the curved surfaces of microgrooves more thoroughly than that of two-dimensional surface roughness parameters.

Study on In-Situ Tool Wear Detection during Micro End Milling Based on Machine Vision.

Most in situ tool wear monitoring methods during micro end milling rely on signals captured from the machining process to evaluate tool wear behavior; accurate positioning in the tool wear region and direct measurement of the level of wear are difficult to achieve. In this paper, an in situ monitoring system based on machine vision is designed and established to monitor tool wear behavior in micro end milling of titanium alloy Ti6Al4V. Meanwhile, types of tool wear zones during micro end milling are discussed and analyzed to obtain indicators for evaluating wear behavior. Aiming to measure such indicators, this study proposes image processing algorithms. Furthermore, the accuracy and reliability of these algorithms are verified by processing the template image of tool wear gathered during the experiment. Finally, a micro end milling experiment is performed with the verified micro end milling tool and the main wear type of the tool is understood via in-situ tool wear detection. Analyzing the measurement results of evaluation indicators of wear behavior shows the relationship between the level of wear and varying cutting time; it also gives the main influencing reasons that cause the change in each wear evaluation indicator.

Cupping alleviates lung injury through the adenosine/A2BAR pathway.

Background: Acute lung injury (ALI) is a serious condition. Inflammation plays a crucial role in the pathogenesis of ALI. Cupping, as a part of traditional Chinese medicine, is still a popular complementary and alternative therapy for a variety of ailments including respiratory diseases. However, reliable scientific data about cupping therapy are scarce. Adenosine, a purine nucleoside produced under metabolic stress by the action of extracellular ectonucleotidases (i.e. CD39 and CD73), can attenuate ALI through the A2BAR receptor. The aim of this study was to investigate the protective effect of cupping in a rat model of ALI and the role of adenosine in it. Methods: Male adult rats were subjected to ALI by intratracheal LPS instillation (0.3 mg/kg). Immediately after intratracheal LPS instillation, vacuum pressure was applied to a sanitized plastic bell cup on the back of the rat by suction for 10 min. Pulmonary injury and inflammation were assessed at 4 h after LPS challenge. The role of adenosine and A2BAR in cupping's protection after LPS instillation were evaluated. Results: Cupping alleviated LPS-induced lung injury, reduced inflammation and inhibited NF-kB activation in rats. Cupping upregulated CD39 and CD73 mRNA expression of the skin tissue at the cupping site and increased circulating levels of adenosine. Administration of PSB1115, a specific adenosine A2BAR receptor antagonist, abolished cupping's beneficial effects in LPS-induced ALI. Conclusions: Cupping attenuates lung inflammation and injury through the adenosine/A2BAR pathway. The current study provides evidence-based information about cupping therapy in ALI.

Preparation of Micro-Pit-Textured PCD Tools and Micro-Turning Experiment on SiCp/Al Composites.

Serious tool wear occurs very often during machining due to the reinforcing phases in the workpiece. In this study, micro-pit-textures were prepared on the surfaces of PCD tools with a nanosecond laser to improve their cutting performance on SiCp/Al composites. The micro-pits were designed with rounded corners to improve the chip flow. The location and size of the texture were determined by analyzing the tool-chip contact area of the non-textured tool. The cutting performance of these textured PCD tools was investigated through orthogonal cutting experiments. It was found that the optimal cutting performance of the textured tools was achieved with the proper distance of the texture from the main cutting edge (35 µm) and the pit spacing (60 µm), aa a result of which the main cutting force reduced by about 14%, and the tool wear and surface adhesion significantly reduced. This texture was then applied in the micro-turning experiments of the PCD tool on the SiCp/Al composites. The cutting force in this experiment reduced by 22%, and the textured tool provided better chip transfer and tool anti-tipping. In this study, the role of SiC particles as a third body between the tool and the chip surface is discussed.

A "flexible" carborane-cored luminogen: variable emission behaviours in aggregates.

The performance of tunable emissions in aggregates is highly desirable but challenging owing to the restricted molecular conformations of luminogens. Herein, we designed and synthesized a new "flexible" luminogen, a carborane-cored compound NAPH, which exhibits variable photophysical behaviours in aggregates, such as aggregation-induced emission, crystallization-induced emission, polymorph-dependent emission, and mechanochromic luminescence. Moreover, the two polymorphs with different emission colors show opposite mechano-responsive luminescence, which is rarely observed for single-component luminescent materials. Both theoretical calculations and photophysical experiments reveal that the carborane-cored luminogen could afford variable conformations. This endows the whole molecule with multiple conformations in aggregates, thus leading to variable emission behaviours. Therefore, the present work provides new access to the construction of multifunctional single-component solid-state luminescent materials.

Reversible Wettability between Underwater Superoleophobicity and Superhydrophobicity of Stainless Steel Mesh for Efficient Oil-Water Separation.

Design and fabrication of smart materials with reversible wettability for oil-water separation have attracted worldwide attention due to the increasingly serious water pollution problem. In this study, a rough oxide coating with micro/nanoscale structures is developed on the 304 stainless steel mesh (SSM) by laser ablation. The smart surface with ethanol immersion and natural drying treatments shows the wetting conversion between underwater superoleophobicity and superhydrophobicity. Based on the wettability transition behavior, both light and heavy oil-water mixtures can be separated with the high separation efficiency. Moreover, after being exposed to various corrosive solutions and high temperatures, the smart surface still shows prominent environmental stability. Switchable surface with excellent properties should be an optimal choice to solve the environmental conditions that need to be addressed urgently.

Circ_PSD3 promotes the progression of papillary thyroid carcinoma via the miR-637/HEMGN axis.

AIMS: In the present study, we aimed to uncover the potential functions of circular RNA (circRNA) pleckstrin and Sec7 domain containing 3 (circ_PSD3) in papillary thyroid carcinoma (PTC) development. MAIN METHODS: The abundance of circ_PSD3, PSD3 messenger RNA (mRNA), microRNA-637 (miR-637) and hemogen (HEMGN; EDAG-1) mRNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Flow cytometry was employed to measure cell cycle progression and cell apoptosis. Western blot assay was used to examine protein expression. The proliferation ability and motility of PTC cells were analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell assays, respectively. The interaction between miR-637 and circ_PSD3 or HEMGN was tested by dual-luciferase reporter assay. Animal experiments were used to explore the role of circ_PSD3 in PTC progression in vivo. KEY FINDINGS: Circ_PSD3 was aberrantly up-regulated in PTC tumor tissues compared with adjacent normal tissues. Circ_PSD3 and HEMGN promoted the cell cycle progression, proliferation and metastasis and impeded the apoptosis of PTC cells. MiR-637 was a direct target of circ_PSD3, and miR-637 directly interacted with HEMGN mRNA in PTC cells. Circ_PSD3 silencing-induced effects in PTC cells were partly attenuated by the addition of anti-miR-637 or HEMGN overexpression plasmid. Circ_PSD3/miR-637/HEMGN regulated the activity of PI3K/Akt signal pathway in PTC cells. Circ_PSD3 silencing inhibited the tumor growth in vivo. SIGNIFICANCE: Circ_PSD3 promoted the progression of PTC through regulating miR-637/HEMGN axis and activating PI3K/Akt signaling. Circ_PSD3/miR-637/HEMGN signaling axis might be a potential target for PTC therapy.