Six-minute walk test distance does not reflect oxygen uptake in patients undergoing maintenance hemodialysis.

AIMS: By evaluating the exercise capacity of patients on maintenance hemodialysis (MHD), this study aimed to determine the representability of a 6-minute walk test (6MWT) distance for the cardiopulmonary endurance capacity of patients on MHD. MATERIALS AND METHODS: The volume of oxygen uptake (VO2) at a respiratory exchange ratio equal to 1 (VO2(RER=1)) was measured during a graded cycling test, and the 6MWT distance was tested in 27 Chinese patients on MHD and 44 age-matched non-MHD subjects (CON). RESULTS: VO2(RER=1) in MHD was lower than the levels of CON (834.44 ± 232.73 and 1,255.08 ± 340.49 mL/min, respectively (t = 5.65, p < 0.001)). The 6MWT distance of MHD was ~ 92 m shorter than that of CON (t = 7.58, p < 0.001). Additionally, a positive linear correlation between 6MWT distance and VO2(RER=1) during the graded cycling test was found in CON (r = 0.57, p < 0.001) but was absent in MHD (r = 0.12, p = 0.582). CONCLUSION: Results indicate a significantly reduced cardiopulmonary endurance capacity and functional walk ability among Chinese patients on MHD. Moreover, 6MWT may not be a proper method to evaluate the cardiopulmonary endurance capacity of patients on MHD due to the absence of a correlation between 6MWT distance and oxygen uptake during exercise.

Erratum: 1-[4-(Di-methyl-amino)-benzyl-idene]-4-o-tolyl-thio-semicarbazide. Corrigendum.

The correspondence address in the paper by Huang et al. [Acta Cryst. (2013), E69, o906-o907] is corrected.[This corrects the article DOI: 10.1107/S1600536813012890.].

1-[4-(Di-methyl-amino)-benzyl-idene]-4-o-tolyl-thio-semicarbazide.

The asymmetric unit of the title compound, C17H20N4S, contains two independent mol-ecules, the main difference between them being the dihedral angles between the benzene rings [19.99 (17) and 9.72 (17)°]. The mol-ecules both have a trans conformation about the C=N double bond and intra-molecular C-H⋯S and N-H⋯N hydrogen bonds are observed in both mol-ecules. In the crystal, mol-ecules are linked by weak N-H⋯S hydrogen bonds with graph-set motif R 2 (2)(8). In each mol-ecule, all but one of the N atoms and both the S atoms are involved in hydrogen bonding.

Timing of continuous renal replacement therapy in patients with acute non-ST-segment elevation myocardial infarction complicated with cardiac and renal insufficiency.

OBJECTIVE: To explore the efficacy of different timing options for continuous renal replacement therapy (CRRT) in patients with acute non-ST-segment elevation myocardial infarction (NSTEMI) and cardiac and renal insufficiency. METHODS: Eighty-eight patients with acute NSTEMI complicated with cardiac and renal insufficiency received PCI treatment after achieving a stable condition and were randomly divided into the control group (n = 44) and the research group (n = 44). The control group was given CRRT after percutaneous coronary intervention (PCI), and the research group was treated with CRRT before and after PCI. The clinical treatment efficacy, cardiac function indexes (left ventricular ejection fraction (LVEF), cardiac output (CO), and left ventricular end diastolic diameter (LVEDD)), renal function indexes (creatinine (Cr), glomerular filtration rate (GFR), neutrophil gelatinase-associated lipocalin (NGAL)), quality of life (QoL) and incidence of major adverse cardiovascular events were compared between the two groups. RESULTS: After treatment, the overall effective rate in the research group was higher than that in the control group (P < 0.05); LVEF, CO, GFR and QoL score were higher, while LVEDD value, creatinine level, NGAL level and the incidence of major adverse cardiovascular events were lower in the research group than in the control group (P < 0.05). CONCLUSION: For patients with acute NSTEMI complicated with cardiac and renal insufficiency, the use of CRRT before and after PCI can effectively ameliorate cardiac and renal function, and significantly improve quality of life with a good prognosis.

Tetra-aqua-tetra-kis{µ(3)-3,3'-[(E,E)-ethane-1,2-diylbis(nitrilo-methyl-idyne)]benzene-1,2-diolato}octa-zinc(II) N,N-dimethyl-formamide hexa-solvate.

The asymmetric unit of the title compound [Zn(8)(C(16)H(12)N(2)O(4))(4)(H(2)O)(4)]·6C(3)H(7)NO, consists of eight Zn(II) cations, four tetra-valent anionic ligands, L(4-) (L(4-) = 3,3'-(1E,1'E)-(ethane-1,2-diylbis(azan-1-yl-1-yl-idene))bis-(methan-1-yl-1-yl-idene)dibenzene-1,2-bis-(olate), four coordinated water mol-ecules and six N,N-dimethyl-formamide solvate mol-ecules. The coordination complex comprises an octa-nuclear Zn(II) unit with its Zn(II) centers coordinated in two discrete distorted square-pyramidal geometries. Four Zn(II) atoms each coordinate to two nitro-gen atoms and two phenolate oxygen atoms from an individual L(4-) ligand and one coordinated water mol-ecule. The other four Zn(II) atoms each bind to five phenolate oxygen atoms from three different L(4-) ligands. In the crystal structure, the Zn(II) complex unit, coordinated water mol-ecules and dimethyl-formamide solvate mol-ecules are linked via O-H⋯O and C-H⋯O hydrogen bonds. Mol-ecules are connected by additional inter-molecular O-H⋯O and C-H⋯O hydrogen bonds, forming an extensive three dimensional framework.

Antitumor activity of a Trans-thiosemicarbazone schiff base palladium (II) complex on human gastric adenocarcinoma cells.

The development of transition-metal-based antitumor drug candidates increases the metallopharmaceuticals study dramatically. Two trans-thiosemicarbazone-based, Schiff base palladium (Pd) (II) complexes, DMABTSPd (TSPd) and DMABPTSPd (PTSPd), were prepared and characterized as described in our previous study. Here, we investigated whether the two complexes have antitumor effect on human gastric adenocarcinoma cell lines, BGC-823 and SGC-7901, compared with normal human gastric mucosal epithelial cell line, Ges-1. The results show that the Pd complex with the bare amino group (DMABTSPd(TSPd)) can inhibit cell viabilities and induce apoptosis in human gastric carcinoma cells, rather than the Pd complex without the bare amino group (DMABPTSPd (PTSPd)). This occurs via a mitochondrial-related pathway by down-regulating the level of Bcl-2 expression and up-regulating the level of Bid expression. Meanwhile, DMABTSPd (TSPd) suppressed tumor growth via a mitochondrial-related pathway in a nude mouse tumor xenograft model derived from BGC-823 cells. These findings demonstrate that DMABTSPd (TSPd) is worthy of further structural optimization and representing a promising Pd complex for the development of a new antitumor therapeutic agent.

Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection.

The distal segment of the long arm of the Thinopyrum intermedium chromosome 7Ai1 carries the barley yellow dwarf virus (BYDV) resistance gene Bdv2. This segment was transferred to the distal region of the long arm of wheat chromosome 7D in the Yw series of translocation lines by using the ph1b mutant to induce homoeologous pairing. To transfer Bdv2 to commercial varieties, we developed two resistance gene-analog polymorphism (RGAP) markers, Tgp-1(350) and Tgp-2(210), and one randomly amplified polymorphic DNA (RAPD) marker, OPD04(1300). The diagnostic fragments of the RGAP marker Tgp-1(350) and the RAPD marker OPD04(1300) were cloned, sequenced and converted into sequence-characterized amplified region (SCAR) markers, named SC-gp1 and SC-D04, respectively. SC-gp1 and SC-D04 were validated based on available translocation lines and segregating F(2) individuals. Our results indicated that the SCAR markers co-segregated with the BYDV resistance associated with Bdv2. Therefore, they can be used as a low-cost, high-throughput alternative to conventional phenotypic screening in wheat-breeding programs exploiting Bdv2. The marker-assisted selection for BYDV resistance was successfully performed in a wheat-breeding program.