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KEY MESSAGE: An alanine to valine mutation of glutamyl-tRNA reductase's 510th amino acid improves 5-aminolevulinic acid synthesis in rice. 5-aminolevulinic acid (ALA) is the common precursor of all tetrapyrroles and plays an important role in plant growth regulation. ALA is synthesized from glutamate, catalyzed by glutamyl-tRNA synthetase (GluRS), glutamyl-tRNA reductase (GluTR), and glutamate-1-semialdehyde aminotransferase (GSAT). In Arabidopsis, ALA synthesis is the rate-limiting step in tetrapyrrole production via GluTR post-translational regulations. In rice, mutations of GluTR and GSAT homologs are known to confer chlorophyll deficiency phenotypes; however, the enzymatic activity of rice GluRS, GluTR, and GSAT and the post-translational regulation of rice GluTR have not been investigated experimentally. We have demonstrated that a suppressor mutation in rice partially reverts the xantha trait. In the present study, we first determine that the suppressor mutation results from a G â A nucleotide substitution of OsGluTR (and an A â V change of its 510th amino acid). Protein homology modeling and molecular docking show that the OsGluTRA510V mutation increases its substrate binding. We then demonstrate that the OsGluTRA510V mutation increases ALA synthesis in Escherichia coli without affecting its interaction with OsFLU. We further explore homologous genes encoding GluTR across 193 plant species and find that the amino acid (A) is 100% conserved at the position, suggesting its critical role in GluTR. Thus, we demonstrate that the gain-of-function OsGluTRA510V mutation underlies suppression of the xantha trait, experimentally proves the enzymatic activity of rice GluRS, GluTR, and GSAT in ALA synthesis, and uncovers conservation of the alanine corresponding to the 510th amino acid of OsGluTR across plant species.
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Proteínas de Arabidopsis , Arabidopsis , Oryza , Alanina/genética , Alanina/metabolismo , Aldeído Oxirredutases , Ácido Aminolevulínico/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Simulação de Acoplamento Molecular , Mutação , Oryza/genética , Oryza/metabolismo , Valina/genética , Valina/metabolismoRESUMO
Chloroplasts and mitochondria are semi-autonomous organelles and have their own genomes (cytoplasmic genomes). Physical radiations (e.g., γ-rays) have been widely used in artificial mutation induction for plant germplasm enhancement and for breeding new cultivars. However, little is known at the genomic level about which kind of cytoplasmic mutations and/or characteristics could be induced in plants. The present study aimed to investigate the type, number, and distribution of inheritable cytoplasmic mutations induced by γ-rays in rice (Oryza sativa L.). Six plants were selected from the 2nd generation (M2) populations after γ-ray (137Cs) irradiation of the rice cultivar Nipponbare, 2 each for the 3 irradiation doses (150, 250, and 350 Gy), and their genomes were sequenced on an Illumina platform. Together with the whole-genome sequencing data of 3 external Nipponbare control plants, single-base substitutions (SBSs) and insertions/deletions (InDels) in chloroplast (cp) and mitochondrial (mt) genomes were identified and analyzed in-depth using bioinformatic tools. The majority of SBSs and InDels identified were background mutations in the 6 M2 plants, and the number of induced mutations varied greatly among the plants. Most induced mutations were present in a heterogeneous state, reflecting the fact that multiple cp and mt copies existed in the progenitor cells. The induced mutations were distributed in different genomic regions in the 6 M2 plants, including exonic regions, but none of them was predicted to cause nonsynonymous mutations or frameshifts. Our study thus revealed, at the genomic level, characteristics of cytoplasmic mutations induced by γ-rays in rice.
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Raios gama/efeitos adversos , Mutação , Oryza/efeitos da radiação , Sequenciamento Completo do Genoma/métodos , Cloroplastos/genética , Cloroplastos/efeitos da radiação , Genoma de Planta/efeitos da radiação , Sequenciamento de Nucleotídeos em Larga Escala , Mitocôndrias/genética , Mitocôndrias/efeitos da radiação , Oryza/genética , Proteínas de Plantas/genética , Proteínas de Plantas/efeitos da radiação , Sementes/genética , Sementes/efeitos da radiaçãoRESUMO
Molybdenum disulfide quantum dots (MoS2 QDs) were chosen as a functional two-dimensional material to improve the separation performance of a traditional C18 column. In this work, MoS2 QDs were synthesized by the combination of sonication and solvothermal treatment of bulk MoS2. The prepared MoS2 QDs were characterized by transmission electron microscope (TEM), Zeta potential measurement, UV-visible absorption and fluorescence spectroscopy. Then, a novel MoS2 QDs embedded C18 (Sil-MoS2-C18) stationary phase was prepared for performing mixed-mode liquid chromatography. The results of elemental analysis (EA), thermogravimetric analysis (TGA), Fourier transform infrared spectrometry (FT-IR), X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM) and Brunauer-Emmett-Teller (BET) measurements indicated the stationary phase was prepared successfully. Five types of compounds including alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), nucleosides and nucleobases, anilines and flavonoids were utilized to evaluate reversed phase, weak cation exchange and hydrophilic interaction of the new column. To a certain extent, the column could achieve separation for different properties of samples on one column, with less organic solvent and shorter time than conventional alkyl and amino columns. Furthermore, the mechanism for separation was studied by investigating effects of mobile phase composition and pH on retentions. In summary, the Sil-MoS2-C18 stationary phase was deemed able to serve the performance of various types of phases, which revealed the prepared mixed-mode column could be potentially applied for the analysis of complex samples. Graphical abstract.
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The correlation between polycyclic aromatic hydrocarbons (PAHs) and organic carbon is an important indicator of the marine environment. In this paper, twenty-six surface sediment samples were collected from the Bohai Sea and the North Yellow Sea as the study area, and the contents of sixteen PAHs ranged from 71.34 ng·g-1 to 240.78 ng·g-1 with an average of 143.26 ng·g-1 by gas chromatography. The source analysis indicated the source of PAHs in the surface sediments of the study area was more complex, and the percentage of combustion sources was higher than that of petroleum sources, which might cause less negative ecological risks. The average content of total organic carbon (TOC) was 5.25%, as determined by automatic potentiometric titration, and the content of TOC was higher in the Bohai Sea than in the North Yellow Sea. TOC was significantly correlated with HWM-PAHs but was not correlated with LWM-PAHs, which proved organic matter was more effective in controlling high-molecular-weight PAHs than low-molecular-weight PAHs in the surface sediments of the Bohai Sea and the North Yellow Sea.
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Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Hidrocarbonetos Policíclicos Aromáticos/análise , Sedimentos Geológicos/química , Poluentes Químicos da Água/análise , Monitoramento Ambiental , ChinaRESUMO
Long-term in situ measurements of the temperature of hydrothermal fluids have great significance in the research of seafloor hydrothermal activities. Herein, we developed a self-powered battery-free temperature logger for measuring and recording the temperature of hydrothermal fluids. A gravity heat pipe made of titanium alloy was employed as the heat-conducting element of the temperature logger to capture heat from a hydrothermal vent and transfer it to the thermoelectric unit. The thermoelectric generator used herein converted the temperature difference into electrical energy to power the circuit of the temperature logger. Numerical analyses and experiments were performed to investigate the performance of the heat pipe and temperature logger. Results show that the temperature logger can realize self-powered starting at a temperature of >76 °C during a tank test. This paper presents a discussion on a new instrument for temperature measurements of deep-sea hydrothermal fluids.
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In order to better understand the migration and accumulation behavior of polycyclic aromatic hydrocarbons (PAHs) in biological chains in the cold environment around the Ardley Island, a variety of biological and penguin manure samples during China's 36th Antarctic Scientific expedition have been collected and studied. A certain difference in PAHs concentration was observed in the environmental samples, and the order of size was as follows: fish > limpets > krill > manure > brown alga > mosses. The percentage of PAHs with different ring numbers in brown alga, moss, and krill was in the following order: three rings > two rings > four rings > five rings > six rings. The proportion of HMW-PAHs in limpets and fish samples was higher than that in brown alga, moss, manure, and krill samples. The main source of PAHs in environmental samples near Ardley Island is oil, which may be due to the development of tourism in Antarctica, the number of ships and human activities around the region. Therefore, it is imperative to strengthen the protection of the ecological environment in the area around Ardley Island.
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Hidrocarbonetos Policíclicos Aromáticos , Animais , Humanos , Hidrocarbonetos Policíclicos Aromáticos/análise , Regiões Antárticas , Esterco/análise , Monitoramento Ambiental , Meio AmbienteRESUMO
Petroleum hydrocarbons are widespread in seawater. As an important sea area in northern China, the content and distribution of petroleum hydrocarbons in seawater need our attention because of the high toxicity and lasting polluting effects on the ecological environment of the Yellow Sea and Bohai Sea. In addition, there are few reports comparing the diversity of oil-degrading bacteria before and after enrichment. Therefore, we collected surface seawater from 10 sites in the Yellow Sea and Bohai Sea in the autumn of 2020 to study the distribution characteristics of total petroleum hydrocarbons (TPH) and the diversity of oil-degrading bacteria. The concentration of TPH was 81.65 µg/L-139.55 µg/L at ten sites in the Bohai Sea and the Yellow Sea, which conformed to the China Grade II water quality standard (GB3097-1997). Moreover, the pristine/phytane (PR/PH) value of most sites was close to 1, indicating that the area was obviously polluted by exogenous petroleum hydrocarbons. We found that oil-degrading bacteria in the seawater of the Yellow Sea and the Bohai Sea had a good degradation effect on C11-C14 short chain alkanes (degradation rate of 59.19-73.22 %) and C1-C4 phenanthrene (degradation rate of 48.19-60.74 %). In terms of the diversity of oil-degrading bacteria, Gammaproteobacteria and Alphaproteobacteria dominated the enriched bacterial communities. Notably, the relative abundance of Alcanivorax changed significantly before and after enrichment. We proposed that surface seawater in the Bohai Sea and Yellow Sea could form oil-degrading bacteria mainly composed of Alcanivorax, which had great potential for oil pollution remediation.
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Poluição por Petróleo , Petróleo , Água do Mar/química , Hidrocarbonetos/metabolismo , Alcanos/metabolismo , Bactérias/metabolismo , Petróleo/análise , China , Biodegradação AmbientalRESUMO
Melatonin (Mel) has previously been reported to effectively alleviate nitrogen-limitation (N-L) stress and thus increase nitrogen-use efficiency (NUE) in several plants, but the underlying mechanism remains obscure. Here, we revealed that OsbZIP79 (BASIC LEUCINE ZIPPER 79) is transcriptionally activated under N-L conditions, and its expression is further enhanced by exogenous Mel. By the combined use of omics, genetics, and biological techniques, we revealed that the OsbZIP79-OsABI5 (ABSCISIC ACID INSENSITIVE 5) module stimulated regulation of reactive oxygen species (ROS) homeostasis and the uptake and metabolism of nitrogen under conditions of indoor nitrogen limitation (1/16 normal level). OsbZIP79 activated the transcription of OsABI5, and OsABI5 then bound to the promoters of target genes, including genes involved in ROS homeostasis and nitrogen metabolism, activating their transcription. This module was also indispensable for upregulation of several other genes involved in abscisic acid catabolism, nitrogen uptake, and assimilation under N-L and Mel treatment, although these genes were not directly transactivated by OsABI5. Field experiments demonstrated that Mel significantly improved rice growth under low nitrogen (L-N, half the normal level) by the same mechanism revealed in the nitrogen-limitation study. Mel application produced a 28.6% yield increase under L-N and thus similar increases in NUE. Also, two OsbZIP79-overexpression lines grown in L-N field plots had significantly higher NUE (+13.7% and +21.2%) than their wild types. Together, our data show that an OsbZIP79-OsABI5 module regulates the rice response to N insufficiency (N limitation or low N), which is important for increasing NUE in rice production.
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Melatonina , Oryza , Melatonina/farmacologia , Melatonina/metabolismo , Ácido Abscísico/metabolismo , Oryza/genética , Oryza/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Nitrogênio/metabolismo , Homeostase/genéticaRESUMO
This work analyzed the distribution characteristics, sources, and corresponding environmental risks of 10 organochlorine pollutants (OCPs) in soil samples from 13 stations on the Fildes Peninsula and Adelaide Island in Antarctica in 2020. Results showed that the total amount of OCP residues in the study area ranged from N.D. to 2.66 ng·g-1 with an average concentration of 0.90 ng·g-1. Six organochlorine pesticides were detected, and the residue level of DDTs was slightly lower than that of HCHs. The residues of HCHs were mainly γ-HCH and those of DDTs were mainly O, P'-DDT. Source analysis revealed that the residues mainly originated from atmospheric transport and human activities. Environmental risk analysis showed that in the study area, the residues of HCHs and DDTs were present at low levels, and their ecological risk was low.
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Hidrocarbonetos Clorados , Praguicidas , Poluentes Químicos da Água , Humanos , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Hidrocarbonetos Clorados/análise , Praguicidas/análise , Medição de Risco , China , DDT/análiseRESUMO
The distribution characteristics, sources, and risk levels of organochlorine pesticides (OCPs) in Ny-Ålesund of the Arctic region during China's 10th Arctic expedition were studied in this paper. Results showed that the concentration of OCPs in the surface soil of the Ny-Ålesund region ranged from 0.59 ng·g-1 to 3.87 ng·g-1, with an average concentration of 2.22 ng·g-1. Source analysis showed that the OCPs in the study area mainly originated from historical residues, and a certain degree of conversion between congeners of hexachlorocyclohexanes and dichlorodiphenyltrichloroethanes in the soil occurred due to environmental factors. Ecological risk assessment indicated that the study area was at a low ecological risk level, but the potential environmental impact of OCPs could not be neglected due to the specificity and sensitivity of the Arctic environment.
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Hidrocarbonetos Clorados , Praguicidas , China , Monitoramento Ambiental , Hidrocarbonetos Clorados/análise , Praguicidas/análise , Medição de Risco , Solo/químicaRESUMO
As a welcomed porous material, covalent organic frameworks (COFs) have many advantages and are widely used in various aspects. Particularly, COFs have aroused great attentions of scientists in chromatographic separation field due to their outstanding advantages, such as high stability, large specific surface area and multiple voids. However, endowing COFs with chirality to construct chiral stationary phase (CSP) function is still facing many challenges. Here, we firstly prepared a ß-cyclodextrin (ß-CD) and covalent organic framework functional silica CSP named as COF@CD@SiO2 by one-pot method to perform high performance liquid chromatography (HPLC) chiral separation. The morphology and structure of the synthesized stationary phase were investigated by a variety of characterization methods including Fourier transform infrared spectrometry (FT-IR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), N2 adsorption experiment, powder X-ray diffraction (XRD) and elemental analysis (EA). The prepared stationary phase realized fast separation of six enantiomers in a short time. The separation mechanism was mainly ascribed to the inclusion complexation of ß-cyclodextrin and the mutli-interaction sites from COFs material. In conclusion, the prepared chiral column can be used to achieve fast separation of enantiomers with good stability and reproducibility. These results can open new avenue for using chiral COFs in liquid chromatographic separation.
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Estruturas Metalorgânicas , beta-Ciclodextrinas , Reprodutibilidade dos Testes , Dióxido de Silício , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Expanded porphyrins represent emerging structures in realizing topological chirality; however, their inherent flexibility has hampered the effective chiral resolution. Herein, we rationally designed a decaphyrin 9, which could be separated into its enantiomers in the free-base form. The enantiomers showed noteworthy chiroptical properties, e.g., the intense circular dichroism response in the visible spectrum, and high absorption dissymmetry factors (gabs) of 0.036 at 618 nm. Theoretical analyses further explained the origin of the high gabs value.
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Porfirinas , Dicroísmo Circular , EstereoisomerismoRESUMO
The bovine serum albumin (BSA) was firstly bonded on the surface of graphene quantum dots (GQDs) functionalized silica as the stationary phase named as BSA@GQDs@SiO2 which can perform hydrophilic interaction between the stationary phase and analytes. Characteristic methods including Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TGA) and scanning electron microscope (SEM) were applied to estimate the chemical bonding results and morphological features of the prepared materials. In the chromatographic evaluation part, the BSA@GQDs@SiO2 column showed excellent separation efficiency for the analysis of hydrophilic compounds involving nucleosides and bases, acids, phenols, quinolones, vitamins and alkaloids, which proved the prepared column can exhibit hydrophilic interaction mode with other interactions including hydrogen bonding and electrostatic interaction. What's more, essential experimental approaches were designed to evaluate the retention properties of the prepared column via comparing with a commercial amino column and a prepared GQDs@SiO2 column. Results indicated the prepared column showed excellent separation ability for different kinds of hydrophilic compounds than commercial and single hydrophilic columns. The study showed that BSA@GQDs@SiO2 stationary phase with double hydrophilic materials will be a potential chromatographic material for analyzing various hydrophilic analytes.
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Grafite , Pontos Quânticos , Grafite/química , Interações Hidrofóbicas e Hidrofílicas , Soroalbumina Bovina , Dióxido de Silício/química , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
In this paper, the characteristics of persistent toxic substances (PTSs) in soil of Ny-Ålesund in the Arctic during the 10th Chinese Arctic (Arctic Ocean) scientific investigation were quantitatively analyzed. The sources and toxicity risks of polycyclic aromatic hydrocarbons (PAHs) in the soil was also analyzed. No obvious spatial distribution of PAHs was identified in the study area. LMW-PAHs are the main PAHs in this region, mainly tricyclic PAHs. The results of characteristic ratio method and PCA showed that the PAHs in soil mainly came from petroleum source and petroleum combustion source, and incomplete combustion of coal and wood, and atmospheric transport contributed to some extent. Ecological risk assessment results showed that the PAHs in soil did not bring toxicity risk, and the possibility of ecological risk was very low in Ny-Ålesund in the Arctic.
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Petróleo , Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , China , Carvão Mineral/análise , Monitoramento Ambiental/métodos , Hidrocarbonetos Policíclicos Aromáticos/análise , Medição de Risco , Solo/química , Poluentes do Solo/análiseRESUMO
High energy ion beams are effective physical mutagens for mutation induction in plants. Due to their high linear energy transfer (LET) property, they are known to generate single nucleotide variations (SNVs) and insertion/deletions (InDels, <50 bp) as well as structural variations (SVs). However, due to the technical difficulties to identify SVs, studies on ion beam induced SVs by genome sequencing have so far been limited in numbers and inadequate in nature, and knowledge of SVs is scarce with regards to their characteristics. In the present study, we identified and validated SVs in six M4 plants (designated as Ar_50, Ar_100, C_150, C_200, Ne_50 and Ne_100 according to ion beam types and irradiation doses), two each induced by argon (40Ar18+), carbon (12C6+) and neon (20Ne10+) ion beams and performed in depth analyses of their characteristics. In total, 22 SVs were identified and validated, consisting of 11 deletions, 1 duplication, and 4 intra-chromosomal and 6 inter-chromosomal translocations. There were several SVs larger than 1 kbp. The SVs were distributed across the whole genome with an aggregation with SNVs and InDels only in the Ne_50 mutants. An enrichment of a 11-bp wide G-rich DNA motif 'GAAGGWGGRGG' was identified around the SV breakpoints. Three mechanisms might be involved in the SV formation, i.e., the expansion of tandem repeats, transposable element insertion, and non-allelic homologous recombination. Put together, the present study provides a preliminary view of SVs induced by Ar, C and Ne ion beam radiations, and as a pilot study, it contributes to our understanding of how SVs might form after ion beam irradiation in rice.
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Aberrações Cromossômicas/efeitos da radiação , Genoma de Planta/efeitos da radiação , Íons Pesados , Mutação , Oryza/efeitos da radiação , Radiação Ionizante , Argônio/química , Carbono/química , Elementos de DNA Transponíveis , Heterozigoto , Recombinação Homóloga , Homozigoto , Mutagênese , Neônio/química , Oryza/genética , Projetos Piloto , Sequências de Repetição em TandemRESUMO
The poly(N-isopropylacrylamide) (NIPAAm) was first polymerized onto the surface of graphene quantum dots (GQDs) functionalized silica as packing materials via reversible addition-fragmentation chain transfer (RAFT) polymerization reaction, which can expand the interaction modes between stationary phase and analytes. A series of characteristic methods were selected to estimate the chemical bonding results of silica, involving Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TGA) and scanning electron microscope (SEM). The prepared column can exhibit reversed-phase and hydrophilic interaction modes, which were demonstrated by the retention of eight kinds of target analytes with different Log P values. The column was then applied to separate banlangen granules and further verified by HPLC tandem time-of-flight mass spectrometry (HPLC/QTOF-MS). In conclusion, Sil-GQDs-PNIPAAm stationary phase improved the analysis range and performance of traditional phases, exhibiting flexible selectivity and application prospect for both hydrophobic and hydrophilic analytes.
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Covalent organic frameworks (COFs) have showed expected potential in chromatographic separation due to unique structure and excellent performance. Nowadays, COF materials applied as chromatographic stationary phases is still in its infancy. Here, we modified COF materials on silica using benzene-1,4,5-tetracarboxylic dianhydride (PMDA) and 1,3,5-tris-(4-aminophenyl)triazine (TAPT) monomers by one-pot synthetic method for performing mixed-mode function, named as SiO2@COF. Five characterization methods including thermogravimetric analysis (TGA), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), elemental analysis (EA) and powder X-ray diffraction (XRD) verified the morphology, structure characteristics and physicochemical properties of the materials. SiO2@COF for performing the separation of polar and nonpolar analytes on high performance liquid chromatography (HPLC) achieved the desired results. Retention mechanisms of the constructed SiO2@COF were researched via observing the effects of mobile phase with retention times. Results exhibited that the prepared stationary phase can provide various interaction modes, including hydrophobic, hydrophilic, hydrogen bonding and π-π interactions. In conclusion, the prepared SiO2@COF stationary phase can execute mixed-mode separation abilities and show potential for complex samples analysis.
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Cromatografia Líquida de Alta Pressão/métodos , Estruturas Metalorgânicas/química , Dióxido de Silício/química , Interações Hidrofóbicas e Hidrofílicas , Estruturas Metalorgânicas/síntese química , Espectroscopia de Infravermelho com Transformada de Fourier , Sulfadiazina/análise , Difração de Raios XRESUMO
Active transposable elements (TEs) have drawn more attention as they continue to create new insertions and contribute to genetic diversity of the genome. However, only a few have been discovered in rice up to now, and their activities are mostly induced by artificial treatments (e.g., tissue culture, hybridization etc.) rather than under normal growth conditions. To systematically survey the current activity of TEs in natural rice accessions and identify rice accessions carrying highly active TEs, the transposon insertion polymorphisms (TIPs) profile was used to identify singleton insertions, which were unique to a single accession and represented the new insertion of TEs in the genome. As a result, 10,924 high-confidence singletons from 251 TE families were obtained, covering all investigated TE types. The number of singletons varied substantially among different superfamilies/families, perhaps reflecting distinct current activity. Particularly, eight TE families maintained potentially higher activity in 3,000 natural rice accessions. Sixty percent of rice accessions were detected to contain singletons, indicating the extensive activity of TEs in natural rice accessions. Thirty-five TE families exhibited potentially high activity in at least one rice accession, and the majority of them showed variable activity among different rice groups/subgroups. These naturally active TEs would be ideal candidates for elucidating the molecular mechanisms underlying the transposition and activation of TEs, as well as investigating the interactions between TEs and the host genome.
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High-energy ion beams are known to be an effective and unique type of physical mutagen in plants. However, no study on the mutagenic effect of argon (Ar) ion beam radiation on rice has been reported. Genome-wide studies on induced mutations are important to comprehend their characteristics for establishing knowledge-based protocols for mutation induction and breeding, which are still very limited in rice. The present study aimed to investigate the mutagenic effect of three ion beams, i.e., Ar, carbon (C) and neon (Ne) on rice and identify and characterize heritable induced mutations by the whole genome sequencing of six M4 plants. Dose-dependent damage effects were observed on M1 plants, which were developed from ion beam irradiated dry seeds of two indica (LH15, T23) and two japonica (DS551, DS48) rice lines. High frequencies of chlorophyll-deficient seedlings and male-sterile plants were observed in all M2 populations (up to ~30% on M1 plant basis); plants from the seeds of different panicles of a common M1 plant appeared to have different mutations; the whole genome-sequencing demonstrated that there were 236-453 mutations in each of the six M4 plants, including single base substitutions (SBSs) and small insertion/deletions (InDels), with the number of SBSs ~ 4-8 times greater than that of InDels; SBS and InDel mutations were distributed across different genomic regions of all 12 chromosomes, however, only a small number of mutations (0-6) were present in exonic regions that might have an impact on gene function. In summary, the present study demonstrates that Ar, C and Ne ion beam radiation are all effective for mutation induction in rice and has revealed at the genome level the characteristics of the mutations induced by the three ion beams. The findings are of importance to the efficient use of ion beam radiation for the generation and utilization of mutants in rice.
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A novel multi-mode and chiral separation stationary phase co-modified with copolymer composed of N-isopropyl acrylamide (NIPAM) and aspartame was synthesized by atom transfer radical polymerization (ATRP) reaction. The synthetic material was evaluated using thermogravimetric analysis (TGA), Fourier transform infrared spectrometry (FT-IR) and elemental analysis (EA). Analytes including hydrophobic, hydrophilic, alkaline and acidic compounds were separated well using the prepared stationary phase named Sil-PPAM-NIPAM. Besides, the separation of chiral compounds proved that the developed column also has the potential of chiral separation ability. In summary, the prepared column possesses excellent hydrophilic interaction, ion exchange, reversed-phase and chiral separation modes during the separation of complex and chiral compounds.