BACKGROUND According to the last 2023
Monkeypox (Mpox) Outbreak Global Map from the Centres for
Disease Control and Prevention (
CDC), more than 100 countries with no Mpox
infection report cases.
Brazil stands out in this group and is the second country with the highest number of cases in the last outbreak. OBJECTIVE To contribute to
knowledge of the
virus infection effects in a cellular model, which is important for
diagnosis infections not yet included in a provider´s
differential diagnosis and for developing viral inhibition
strategies.
METHODS We describe a
virus isolation protocol for a
human clinical sample from a
patient from
Brazil, the viral
growth in a
cell model through plaque forming units (PFU) assay,
reverse transcriptase polymerase chain reaction (RT-PCR) and
transmission electron microscopy (TEM). FINDINGS We follow the viral isolation in
Vero cell culture from a Mpox positive clinically diagnosed sample and show the
infection effects on
cellular structures using a TEM. MAIN CONCLUSIONS
Understanding the impact of viral
growth on
cellular structures and its replication
kinetics may offer better
strategies for the development of
new drugs with
antiviral properties.