Genes encoding nitrilase-like proteins from tobacco.
DNA Res
; 7(5): 283-9, 2000 Oct 31.
Article
em En
| MEDLINE
| ID: mdl-11089910
ABSTRACT
Nitrilase (nitrile aminohydrolase, EC 3.5.5.1) catalyzes the hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA). Arabidopsis thaliana genome has four nitrilase genes (NIT1, NIT2, NIT3 and NIT4). Three (NIT1, NIT2 and NIT3) of the four genes have high similarity. We have cloned two NIT4 homologs (TNIT4A and TNIT4B) from tobacco (Nicotiana tabacum). Genomic Southern hybridization, among other experiments, strongly suggests that tobacco has NIT4 homologs but not NIT1 to NIT3 homologs. Introduction of Arabidopsis NIT2 into tobacco conferred IAN-mediated growth inhibition, probably due to hydrolysis of IAN to IAA, while ectopic expression of TNIT4A had little effect on the sensitivity of transgenic plants to IAN. Nitrilase activity of TNIT4 proteins is discussed.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Plantas Tóxicas
/
Nicotiana
/
Genes de Plantas
/
Aminoidrolases
Idioma:
En
Revista:
DNA Res
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão