Salmonella vaccines secreting measles virus epitopes induce protective immune responses against measles virus encephalitis.
Microbes Infect
; 2(14): 1687-92, 2000 Nov.
Article
em En
| MEDLINE
| ID: mdl-11137042
ABSTRACT
In the present study we describe a live vaccine against measles virus (MV) infection on the basis of attenuated Salmonella typhimurium aroA secreting MV antigens via the Escherichia coli alpha-hemolysin secretion system. Two well-characterized MV epitopes, a B-cell epitope of the MV fusion protein (amino acids 404-414) and a T-cell epitope of the MV nucleocapsid protein (amino acids 79-99) were fused as single or repeating units to the C-terminal secretion signal of the E. coli hemolysin and expressed in secreted form by the attenuated S. typhimurium aroA SL7207. Immunization of MV-susceptible C3H mice revealed that S. typhimurium SL7207 secreting these antigens provoked a humoral and a cellular MV-specific immune response, respectively. Mice vaccinated orally with a combination of both recombinant S. typhimurium strains showed partial protection against a lethal MV encephalitis after intracerebral challenge with a rodent-adapted, neurotropic MV strain.
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Base de dados:
MEDLINE
Assunto principal:
Salmonella
/
Panencefalite Esclerosante Subaguda
/
Vacinas Virais
/
Epitopos de Linfócito T
/
Epitopos de Linfócito B
/
Vírus do Sarampo
Limite:
Animals
Idioma:
En
Revista:
Microbes Infect
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
MICROBIOLOGIA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Alemanha