Angular distribution of light scattered by single biological cells and oriented particle agglomerates.
Appl Opt
; 42(31): 6388-97, 2003 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-14649283
ABSTRACT
We used a flow cytometer together with an intensified CCD camera to record spatially resolved light scattering from micrometer-sized single particles and single oriented particle agglomerates. Experimental differential cross sections of an oriented dumbbell made from two identical polystyrene spheres were compared with theoretical values calculated within the discrete dipole approximation, and good agreement was achieved. Furthermore, characteristic two-dimensional patterns of the scattered-light intensity were recorded for single blood cells, yielding information on the cells' shape and volume. Besides flow cytometry, we observed and analyzed differential light scatter of particle clusters of known size, shape, and orientation located within an optical trap.
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Base de dados:
MEDLINE
Assunto principal:
Gravação em Vídeo
/
Algoritmos
/
Interpretação de Imagem Assistida por Computador
/
Eritrócitos
/
Citometria de Fluxo
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Leucócitos
/
Micromanipulação
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Appl Opt
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Alemanha