A genome annotation-driven approach to cloning the human ORFeome.
Genome Biol
; 5(10): R84, 2004.
Article
em En
| MEDLINE
| ID: mdl-15461802
ABSTRACT
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genoma Humano
/
Fases de Leitura Aberta
/
Clonagem Molecular
/
Proteoma
/
Genômica
Limite:
Humans
Idioma:
En
Revista:
Genome Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Reino Unido