Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges.
Electrophoresis
; 27(9): 1758-67, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16586414
ABSTRACT
Generation 5 ethylenediamine (EDA)-cored poly(amidoamine) (PAMAM) dendrimers (E5, E denotes the EDA core and 5 the generation number) with different degrees of acetylation and carboxylation were synthesized and used as a model system to investigate the effect of charge and the influence of dendrimer surface modifications on electrophoretic mobility (EM) and molecular distribution. The surface-modified dendrimers were characterized by size-exclusion chromatography, 1H NMR, MALDI-TOF-MS, PAGE, and CE. The focus of our study was to determine how EM changes as a function of particle charge and molecular mass, and how the molecular distribution changes due to surface modifications. We demonstrate that partially modified dendrimers have much broader migration peaks than those of fully surface functionalized or unmodified E5 dendrimers due to variations in the substitution of individual dendrimer surfaces. EM decreased nonlinearly with increases in surface acetylation for both PAMAM acetamides and PAMAM succinamic acids, indicating a complex migration activity in CE separations that is not solely due to charge/mass ratio changes. These studies provide new insights into dendrimer properties under an electric field, as well as into the characterization of dendrimer-based materials being developed for medical applications.
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Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Eletroforese em Gel de Poliacrilamida
/
Dendrímeros
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos