[Correlation between MMP-2 activation and MT1-MMP mRNA expression in thymic epithelial tumors].
Zhonghua Bing Li Xue Za Zhi
; 35(1): 18-23, 2006 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-16608644
ABSTRACT
OBJECTIVE:
To study the relationship between activation of pro-MMP-2 and expression of matrix metalloproteinases (MMP)-2, MT1-MMP and tissue inhibitor of metalloproteinases (TIMP)-2 mRNA in thymoma and thymic carcinoma; and to study the molecular mechanism of invasion and metastasis of thymic epithelial tumors.METHODS:
Fresh tissue specimens of thymoma, thymic carcinoma and normal thymus were included. The mRNA expression of MMP-2, MT1-MMP and TIMP-2 were analyzed by real-time reverse transcription polymerase chain reaction. The pro-MMP-2 activation ratio and its localization were determined by gelatin zymography and film in-situ gelatin-Zymography, respectively. Correlation of mRNA expression of MMP-2, MT1-MMP and TIMP-2 was investigated in tumors with different histological subtypes and clinical stages.RESULTS:
There were no significant differences in the expressions of MMP-2, MT1-MMP and TIMP-2 mRNA between I and II stage or III and IV stage thymomas (P > 0.05). However, significant differences of the expressions were observed between three tumor groups I-II stage, III-IV stage and thymic carcinomas (P < 0.005), and between three histological subtypes AB-B1 (lymphocyte-rich and mixed types), B2-B3 (cortical and predominantly polygonal cells types) and thymic carcinomas (P < 0.05). Expression levels of MT1-MMP and TIMP-2 mRNA were correlated with pro-MMP-2 activation ratio (Spearman rank correlation r = 0.7235, r = 0.7647, P < 0.005). The expression of MMP-9 did not show significant differences between thymomas and thymic carcinomas.CONCLUSIONS:
MMP-2, MT1-MMP and TIMP-2 mRNA expression levels are correlated with the histologic subtypes and clinical stages of thymoma. The mRNA expressions of MT1-MMP and TIMP-2 are correlated with the activation ratio of pro-MMP-2. It is speculated that upregulation of MT1-MMP gene expression may induce an activation of pro-MMP-2 through TIMP-2.
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Base de dados:
MEDLINE
Assunto principal:
Timoma
/
Neoplasias do Timo
/
Carcinoma de Células Escamosas
/
Metaloproteinase 2 da Matriz
/
Metaloproteinases da Matriz
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Bing Li Xue Za Zhi
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China