Involvement of Sp-1 in the regulation of the Id-1 gene during trophoblast cell differentiation.
Placenta
; 28(2-3): 192-8, 2007.
Article
em En
| MEDLINE
| ID: mdl-16638616
ABSTRACT
Id-1, a member of the helix-loop-helix transcription factor family, inhibits the differentiation of Rcho-1 cells, which were derived from rat choriocarcinoma and consist of trophoblast stem cells. Id-1 is expressed at a high level in undifferentiated trophoblast stem cells and then down-regulated during early differentiation, and is thought to be a key regulator in the trophoblast giant-cell differentiation pathway. In this study, we analyzed the signaling mechanism regulating the high expression levels of Id-1 in undifferentiated Rcho-1 cells. Promoter deletion analysis revealed that a 31-bp sequence (Box-2 region), located between -200 and -169bp in the Id-1 promoter is necessary for the promoter activity. Electrophoretic mobility shift assays and DNA affinity precipitation assays showed that Box-2-binding activity was decreased during differentiation and that Sp-1 protein bound to this sequence. The protein level of Sp-1 was decreased during the differentiation. These results suggest that the Sp-1 protein level may regulate the Box-2-binding activity and the trophoblast giant-cell differentiation.
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Base de dados:
MEDLINE
Assunto principal:
Trofoblastos
/
Diferenciação Celular
/
Fator de Transcrição Sp1
/
Proteína 1 Inibidora de Diferenciação
Limite:
Animals
Idioma:
En
Revista:
Placenta
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão