Crystallization and preliminary X-ray diffraction data of the rat histone H1(0) globular domain.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 63(Pt 5): 403-5, 2007 May 01.
Article
em En
| MEDLINE
| ID: mdl-17565181
ABSTRACT
The linker histones H1 are a family of lysine-rich proteins that associate with the stretch of DNA that enters and exits the nucleosome. The linker histones facilitate the compaction and condensation of chromatin. The globular domain of histone H1(0), a specific subtype of histone H1, was crystallized at 288 K using the microbatch under silicone oil method with potassium phosphate as a precipitating agent. Diffraction data were collected to a resolution of 1.98 A. The crystal belongs to the trigonal space group P3(1)21, with unit-cell parameters a = 54.13, b = 54.13, c = 71.99 A, and contains one molecule per asymmetric unit. The V(M) value and solvent content were calculated to be 3.04 A3 Da(-1) and 59.6%, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Histonas
Limite:
Animals
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão