Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1.
Mol Immunol
; 46(5): 917-26, 2009 Feb.
Article
em En
| MEDLINE
| ID: mdl-18952295
ABSTRACT
Human anti-V3 monoclonal antibodies (mAbs) generated from HIV-1 infected individuals display diversity in the range of their cross-neutralization that may be related to their immunogenetic background. The study of the immunoglobulin (Ig) variable region gene usage of heavy chains have shown a preferential usage of the VH5-51 gene segment which was detected in 35% of 51 human anti-V3 mAbs. In contrast, human mAbs against other envelope regions of HIV-1 (anti-Env), including the CD4-binding domain, the CD4-induced epitope, and gp41 preferentially used the VH1-69 gene segment, and none of them used the VH5-51 gene. Furthermore, the usage of the VH4 family by anti-V3 mAbs was restricted to only one gene segment, VH4-59, while the VH3 gene family was used at a significantly lower frequency by all of the analyzed anti-HIV-1 mAbs. Multivariate analysis showed that usage of VH gene segments was significantly different between anti-V3 and anti-Env mAbs, and compared to antibodies from healthy subjects. In addition, the anti-V3 mAbs preferentially used the JH3 and D2-15 gene segments. The preferential usage of selected Ig gene segments and the characteristic pattern of Ig gene usage by anti-V3 mAbs can be related to the conserved structure of the V3 region.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Região Variável de Imunoglobulina
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Proteína gp41 do Envelope de HIV
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Infecções por HIV
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HIV-1
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Anticorpos Monoclonais
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Anticorpos Antivirais
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Especificidade de Anticorpos
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Epitopos
Limite:
Humans
Idioma:
En
Revista:
Mol Immunol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos