Application of SYPRO Ruby- and Flamingo-stained polyacrylamide gels to Western blot analysis.
Anal Biochem
; 397(2): 262-4, 2010 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-20301812
ABSTRACT
Western blots are widely used for analysis of the expression levels of specific proteins. Blotting is conducted after sodium dodecyl sulfate or native polyacrylamide gel electrophoresis without staining the gel. However, when it is necessary to analyze the gel, duplicate polyacrylamide gels (one of which is stained) usually must be prepared, leading to the consumption of precious sample. Thus, we have developed a convenient and efficient Western blot method using a stained gel. This simple modification should be beneficial for the analysis of samples that are limited in quantity and/or samples for which the stained gel serves as the loading control.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Western Blotting
/
Proteínas rab5 de Ligação ao GTP
/
Eletroforese em Gel de Poliacrilamida
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão