Stress-activated dendritic cells interact with CD4+ T cells to elicit homeostatic memory.
Eur J Immunol
; 40(6): 1628-38, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-20391436
ABSTRACT
Evidence is presented that thermal or oxidizing stress-activated DC interact with CD4(+) T cells to induce and maintain a TCR-independent homeostatic memory circuit. Stress-activated DC expressed endogenous intra-cellular and cell surface HSP70. The NF-kappaB signalling pathway was activated and led to the expression of membrane-associated IL-15 molecules. These interacted with the IL-15 receptor complex on CD4(+) T cells, thus activating the Jak3 and STAT5 phosphorylation signalling pathway to induce CD40 ligand expression, T-cell proliferation and IFN-gamma production. CD40 ligand on CD4(+) T cells in turn re-activated CD40 molecules on DC, inducing DC maturation and IL-15 expression thereby maintaining the feedback circuit. The proliferating CD4(+) T cells were characterized as CD45RA(-) CD62L(+) central memory cells, which underwent homeostatic proliferation. The circuit is independent of antigen and MHC-class-II-TCR interaction as demonstrated by resistance to TCR inhibition by ZAP70 inhibitor or MHC-class II antibodies. These findings suggest that stress can activate a DC-CD4(+) T-cell interacting circuit, which may be responsible for maintaining a homeostatic antigen-independent memory.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
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Células Dendríticas
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Ativação Linfocitária
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Linfócitos T CD4-Positivos
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Memória Imunológica
Limite:
Humans
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido