High yield sample preconcentration using a highly ion-conductive charge-selective polymer.
Anal Chem
; 82(14): 6287-92, 2010 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-20575520
ABSTRACT
The development and analysis of a microfluidic sample preconcentration system using a highly ion-conductive charge-selective polymer [poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid)] is reported. The preconcentration is based on the phenomenon of concentration polarization which develops at the boundaries of the poly-AMPS with buffer solutions. A negatively charged polymer, poly-AMPS, positioned between two microchannels efficiently extracts cations through its large cross section, resulting in efficient anion sample preconcentration. The present work includes the development of a robust polymer that is stable over a wide range of buffers with varying chemical compositions. The sample preconcentration effect remains linear to over 3 mM (0.15 pmol) and 500 microM (15 fmol) for fluorescein and TRITC-tagged albumin solutions, respectively. The system can potentially be used for concentrating proteins on microfluidic devices with subsequent analysis for proteomic applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Técnicas Analíticas Microfluídicas
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos