Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.
J Clin Microbiol
; 48(9): 3392-4, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20592150
ABSTRACT
We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Fúngico
/
Reação em Cadeia da Polimerase
/
Mucorales
/
Mucormicose
/
Micologia
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
República Tcheca