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High glucose upregulation of early-onset Parkinson's disease protein DJ-1 integrates the PRAS40/TORC1 axis to mesangial cell hypertrophy.
Das, Falguni; Dey, Nirmalya; Venkatesan, Balachandar; Kasinath, Balakuntalam S; Ghosh-Choudhury, Nandini; Choudhury, Goutam Ghosh.
Afiliação
  • Das F; Department of Medicine, University of Texas Health Science Center at San Antonio, TX, 78229­3900, USA.
Cell Signal ; 23(8): 1311-9, 2011 Aug.
Article em En | MEDLINE | ID: mdl-21426932
The Akt kinase signaling pathway is frequently deregulated in many human diseases including cancer, autoimmune disease and diabetes. In nephropathy, associated with diabetes, increased Akt signal transduction results in glomerular especially mesangial cell hypertrophy. The mechanism of Akt activation by elevated glucose is poorly understood. The oncogene DJ-1 prevents oxidative damage and apoptosis of dopaminergic neurons in animal models of Parkinson's disease and in culture. We identified DJ-1 to increase in response to high glucose in renal glomerular mesangial cells concomitant with an increase in phosphorylation of Akt in a time-dependent manner. Plasmid-derived overexpression as well as downregulation of DJ-1 by siRNA showed the requirement of this protein in high glucose-stimulated Akt phosphorylation. The tumor suppressor protein PTEN acts as a negative regulator of Akt activation. Interestingly, DJ-1 was associated with PTEN and this interaction was significantly increased in response to high glucose. High glucose-induced increase in DJ-1 promoted phosphorylation of the PRAS40, a negative regulator of TORC1 kinase activity, resulting in activating and inactivating phosphorylation of S6 kinase and 4EBP-1, respectively. Furthermore, DJ-1 increased protein synthesis and hypertrophy of mesangial cells. Our results provide evidence for a unique mechanism whereby DJ-1 induces Akt/PRAS40/TORC1-mediated hypertrophy in response to high glucose.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Adaptadoras de Transdução de Sinal / Células Mesangiais / Proteínas Associadas aos Microtúbulos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Cell Signal Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Adaptadoras de Transdução de Sinal / Células Mesangiais / Proteínas Associadas aos Microtúbulos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Cell Signal Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos