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Both adiponectin and interleukin-10 inhibit LPS-induced activation of the NF-κB pathway in 3T3-L1 adipocytes.
Lira, Fábio Santos; Rosa, José Cesar; Pimentel, Gustavo Duarte; Seelaender, Marília; Damaso, Ana Raimunda; Oyama, Lila Missae; do Nascimento, Claudia Oller.
Afiliação
  • Lira FS; Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, Brazil. fabioslira@gmail.com
Cytokine ; 57(1): 98-106, 2012 Jan.
Article em En | MEDLINE | ID: mdl-22047972
ABSTRACT
Adiponectin and interleukin 10 (IL-10) are adipokines that are predominantly secreted by differentiated adipocytes and are involved in energy homeostasis, insulin sensitivity, and the anti-inflammatory response. These two adipokines are reduced in obese subjects, which favors increased activation of nuclear factor kappa B (NF-κB) and leads to elevation of pro-inflammatory adipokines. However, the effects of adiponectin and IL-10 on NF-κB DNA binding activity (NF-κBp50 and NF-κBp65) and proteins involved with the toll-like receptor (TLR-2 and TLR-4) pathway, such as MYD88 and TRAF6 expression, in lipopolysaccharide-treated 3T3-L1 adipocytes are unknown. Stimulation of lipopolysaccharide-treated 3T3-L1 adipocytes for 24h elevated IL-6 levels; activated the NF-κB pathway cascade; increased protein expression of IL-6R, TLR-4, MYD88, and TRAF6; and increased the nuclear activity of NF-κB (p50 and p65) DNA binding. Adiponectin and IL-10 inhibited the elevation of IL-6 levels and activated NF-κB (p50 and p65) DNA binding. Taken together, the present results provide evidence that adiponectin and IL-10 have an important role in the anti-inflammatory response in adipocytes. In addition, inhibition of NF-κB signaling pathways may be an excellent strategy for the treatment of inflammation in obese individuals.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Lipopolissacarídeos / NF-kappa B / Interleucina-10 / Adipócitos / Adiponectina Limite: Animals Idioma: En Revista: Cytokine Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Lipopolissacarídeos / NF-kappa B / Interleucina-10 / Adipócitos / Adiponectina Limite: Animals Idioma: En Revista: Cytokine Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil