Gene expression in livers of BALB/C and C57BL/6J mice fed a high-fat diet.
Toxicol Pathol
; 40(1): 71-82, 2012.
Article
em En
| MEDLINE
| ID: mdl-22105644
ABSTRACT
We previously demonstrated that high-fat diet (HFD)-induced hepatic lipid accumulation is more severe in BALB/c mice than in C57BL/6J (B6) mice. To understand the changes in liver metabolism, we studied blood chemistry, gene expression, and histopathological changes of the liver in nine-week HFD-fed BALB/c and B6 mice and one- or four-week HFD-fed BALB/c mice. Serum total cholesterol and triglyceride levels were significantly increased in all HFD-fed groups, and one- and four-week HFD-fed BALB/c groups, respectively. Histopathology revealed that vacuolation of hepatocytes was severe in nine-week HFD-fed BALB/c mice, although it was less severe in the other groups. Microarray analysis of mRNA expression of nine-week HFD-fed BALB/c mice showed up-regulation of genes involved in fatty acid uptake and biosynthesis, such as Cd36, Acaca, Acly, and Fasn. Some changes were observed in the one- and four-week HFD-fed BALB/c groups and the nine-week HFD-fed B6 group, however these changes in mRNA expression were not so marked. In conclusion, the fatty accumulation observed in BALB/c mice may be caused, at least in part, by up-regulation of fatty acid uptake and biosynthesis. Cd36, Acaca, Acly and Fasn may be involved in these metabolic processes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Gorduras na Dieta
/
Dieta Hiperlipídica
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Toxicol Pathol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão