The alternative lengthening of telomeres pathway may operate in non-neoplastic human cells.
J Pathol
; 226(3): 509-18, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-22250043
ABSTRACT
The alternative lengthening of telomeres (ALT) mechanism represents an alternative to the enzyme telomerase in the maintenance of mammalian telomeres in 25-60% of sarcomas and a minority of carcinomas (about 5-15%). ALT-positive cells are distinguished by long and heterogeneous telomere length distributions by terminal restriction fragment (TRF) Southern blotting. Another diagnostic marker of ALT is discrete nuclear co-localized signals of telomeric DNA and the promyelocytic leukaemia protein (PML), referred to as ALT-associated PML bodies (APBs). Recently, we detected smaller sized co-localized PML and telomere DNA (APB-like) bodies in endothelial cells adjacent to astrocytoma tumour cells in situ. In this study, we examined a wide variety of non-neoplastic tissues, and report that co-localized signals of PML and telomere DNA are present in endothelial, stromal, and some epithelial cells. Co-localized signals of PML and telomere DNA showed an increased frequency in non-neoplastic cells with DNA damage. These results suggest that a mechanism similar to that in ALT-positive tumours also operates in non-neoplastic cells, which may be activated by DNA damage.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Telômero
/
Células Estromais
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Células Endoteliais
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Células Epiteliais
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Homeostase do Telômero
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adult
/
Aged
/
Aged80
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Humans
/
Middle aged
Idioma:
En
Revista:
J Pathol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Nova Zelândia