Stereotactic intracranial implantation and in vivo bioluminescent imaging of tumor xenografts in a mouse model system of glioblastoma multiforme.
J Vis Exp
; (67)2012 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-23051742
ABSTRACT
Glioblastoma multiforme (GBM) is a high-grade primary brain cancer with a median survival of only 14.6 months in humans despite standard tri-modality treatment consisting of surgical resection, post-operative radiation therapy and temozolomide chemotherapy. New therapeutic approaches are clearly needed to improve patient survival and quality of life. The development of more effective treatment strategies would be aided by animal models of GBM that recapitulate human disease yet allow serial imaging to monitor tumor growth and treatment response. In this paper, we describe our technique for the precise stereotactic implantation of bio-imageable GBM cancer cells into the brains of nude mice resulting in tumor xenografts that recapitulate key clinical features of GBM. This method yields tumors that are reproducible and are located in precise anatomic locations while allowing in vivo bioluminescent imaging to serially monitor intracranial xenograft growth and response to treatments. This method is also well-tolerated by the animals with low perioperative morbidity and mortality.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transplante Heterólogo
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Neoplasias Encefálicas
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Técnicas Estereotáxicas
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Glioblastoma
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Medições Luminescentes
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Transplante de Neoplasias
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos