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A strategy to capture and characterize the synaptic transcriptome.
Puthanveettil, Sathyanarayanan V; Antonov, Igor; Kalachikov, Sergey; Rajasethupathy, Priyamvada; Choi, Yun-Beom; Kohn, Andrea B; Citarella, Mathew; Yu, Fahong; Karl, Kevin A; Kinet, Maxime; Morozova, Irina; Russo, James J; Ju, Jingyue; Moroz, Leonid L; Kandel, Eric R.
Afiliação
  • Puthanveettil SV; Department of Neuroscience and Howard Hughes Medical Institute, Columbia University, New York, NY 10027, USA. sputhanv@scripps.edu
Proc Natl Acad Sci U S A ; 110(18): 7464-9, 2013 Apr 30.
Article em En | MEDLINE | ID: mdl-23589870
Here we describe a strategy designed to identify RNAs that are actively transported to synapses during learning. Our approach is based on the characterization of RNA transport complexes carried by molecular motor kinesin. Using this strategy in Aplysia, we have identified 5,657 unique sequences consisting of both coding and noncoding RNAs from the CNS. Several of these RNAs have key roles in the maintenance of synaptic function and growth. One of these RNAs, myosin heavy chain, is critical in presynaptic sensory neurons for the establishment of long-term facilitation, but not for its persistence.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aplysia / Sinapses / Perfilação da Expressão Gênica / Transcriptoma Limite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Aplysia / Sinapses / Perfilação da Expressão Gênica / Transcriptoma Limite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Estados Unidos