Highly water-soluble BODIPY-based fluorescent probe for sensitive and selective detection of nitric oxide in living cells.
ACS Appl Mater Interfaces
; 5(10): 4107-12, 2013 May 22.
Article
em En
| MEDLINE
| ID: mdl-23614822
A highly water-soluble BODIPY dye bearing electron-rich o-diaminophenyl groups at 2,6-positions was prepared as a highly sensitive and selective fluorescent probe for detection of nitric oxide (NO) in living cells. The fluorescent probe displays an extremely weak fluorescence with fluorescence quantum yield of 0.001 in 10 mM phosphate buffer (pH 7.0) in the absence of NO as two electron-rich o-diaminophenyl groups at 2,6-positions significantly quench the fluorescence of the BODIPY dye via photoinduced electron transfer mechanism. The presence of NO in cells enhances the dye fluorescence dramatically. The fluorescent probe demonstrates excellent water solubility, membrane permeability, and compatibility with living cells for sensitive detection of NO.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos de Boro
/
Água
/
Corantes Fluorescentes
/
Óxido Nítrico
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos