Selective fluorimetric method for the determination of histamine in seafood samples based on the concept of zone fluidics.
Anal Chim Acta
; 778: 48-53, 2013 May 17.
Article
em En
| MEDLINE
| ID: mdl-23639398
ABSTRACT
In the present article we report our results on the development of a selective automated method for the determination of histamine in seafood using the concept of zone fluidics. The method is based on the sequential on-line reaction of the analyte with o-phthalaldehyde in the absence of a nucleophilic reagent, followed by acidification. The careful selection of the chemical and instrumental variables enabled the determination of the analyte with adequate sensitivity at the low micromolar level and with specificity against other biogenic amines and amino acids such as histidine. The LOD was 0.05 µmol L(-1) (0.6 mg kg(-1)) and linearity was obeyed in the range of 0.5-15 µmol L(-1) (5.5-170 mg kg(-1)). The proposed method offers a satisfactory sampling rate of 15 h(-1) and adequate accuracy and precision for the analysis of seafood products after minimum sample preparation and without employing a separation technique.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histamina
/
Alimentos Marinhos
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Grécia