Generation of transgenic rats through induced pluripotent stem cells.
J Biol Chem
; 288(38): 27150-27158, 2013 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-23926100
ABSTRACT
The rat is an important animal model for human disease research. Using inhibitors of glycogen synthase kinase 3 and MAPK signaling pathways, rat embryonic stem cells and rat induced pluripotent stem cells (riPSCs) have been derived. However, unlike rat embryonic stem cells, germ line competent riPSCs have only been derived from Wistar rats at low efficiency. Here, we found that an optimized induction medium containing knock-out serum replacement and vitamin C improved the rate and efficiency of riPSCs generation from Dark Agouti rat fibroblasts and Sertoli cells. riPSCs maintained an undifferentiated status for >30 passages and could differentiate into various cells types including germ cells when injected into rat blastocysts. Moreover, transgenic riPSCs could be generated through the PiggyBac transposon, which could be used to generate transgenic rats through germ line transmission. riPSCs can be used as a novel tool in genetic and genomic studies of the rat.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células de Sertoli
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Blastocisto
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Células-Tronco Pluripotentes Induzidas
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Fibroblastos
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
J Biol Chem
Ano de publicação:
2013
Tipo de documento:
Article