CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.
Genetics
; 195(3): 1187-91, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23979586
ABSTRACT
The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mutagênese Sítio-Dirigida
/
Caenorhabditis elegans
/
Sistemas CRISPR-Cas
Limite:
Animals
Idioma:
En
Revista:
Genetics
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Holanda