West Nile virus genome with glycosylated envelope protein and deletion of alpha helices 1, 2, and 4 in the capsid protein is noninfectious and efficiently secretes subviral particles.
J Virol
; 87(23): 13063-9, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-24049184
ABSTRACT
Flavivirus genomes with deletions in the capsid (C) gene are attractive vaccine candidates, as they secrete highly immunogenic subviral particles (SVPs) without generating infectious virus. Here, we report that cytomegalovirus promoter-driven cDNA of West Nile virus Kunjin (KUNV) containing a glycosylation motif in the envelope (E) gene and a combined deletion of alpha helices 1, 2, and 4 in C produces significantly more SVPs than KUNV cDNAs with nonglycosylated E and various other deletions in C.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus do Nilo Ocidental
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Proteínas do Envelope Viral
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Proteínas do Capsídeo
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Liberação de Vírus
Limite:
Humans
Idioma:
En
Revista:
J Virol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Austrália