Proteomic studies of isolated lipid droplets from bacteria, C. elegans, and mammals.
Methods Cell Biol
; 116: 1-14, 2013.
Article
em En
| MEDLINE
| ID: mdl-24099284
Lipid droplets (LDs) are an intracellular organelle, consisting of a neutral lipid core covered by a monolayer of phospholipids and proteins. It primarily mediates lipid storage, metabolism, and transportation. Recently, research of LDs has emerged as a rapidly developing field due to the strong linkage between ectopic lipid accumulation and metabolic syndromes. Recently, more than 30 proteomic studies of isolated LDs have identified many important LD proteins that have highlighted and have also predicted the potential biological roles of the organelle, motivating the field to develop quite rapidly. This chapter summarizes methods used in proteomic studies for three representative species reported and discusses their advantages and disadvantages. We believe that this chapter provides useful information and methods for future LD proteomic studies especially for LDs in other species.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Corpos de Inclusão
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Proteômica
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Metabolismo dos Lipídeos
Limite:
Animals
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China