Differential expression of αB-crystallin causes maturation-dependent susceptibility of oligodendrocytes to oxidative stress.
BMB Rep
; 46(10): 501-6, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-24148771
Oligodendrocyte precursor cells (OPCs) are most susceptible to oxidative stress in the brain. However, the cause of differences in susceptibility to oxidative stress between OPCs and mature oligodendrocytes (mOLs) remains unclear. Recently, we identified in vivo that αB-crystallin (aBC) is expressed in mOLs but not in OPCs. Therefore, we examined in the present study whether aBC expression could affect cell survival under oxidative stress induced by hydrogen peroxide using primary cultures of OPCs and mOLs from neonatal rat brains. Expression of aBC was greater in mOLs than in OPCs, and the survival rate of mOLs was significantly higher than that of OPCs under oxidative stress. Suppression of aBC by siRNA transfection resulted in a decrease in the survival rate of mOLs under oxidative stress. These data suggest that higher susceptibility of OPCs than mOLs to oxidative stress is due, at least in part, to low levels of aBC expression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligodendroglia
/
Cristalinas
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
BMB Rep
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article