Your browser doesn't support javascript.
loading
Amifostine preserves osteocyte number and osteoid formation in fracture healing following radiotherapy.
Donneys, Alexis; Tchanque-Fossuo, Catherine N; Blough, Jordan T; Nelson, Noah S; Deshpande, Sagar S; Buchman, Steven R.
Afiliação
  • Donneys A; Research Fellow, Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, MI.
  • Tchanque-Fossuo CN; Research Fellow, Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, MI.
  • Blough JT; Research Assistant, Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, MI.
  • Nelson NS; Research Fellow, Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, MI.
  • Deshpande SS; Research Fellow, Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, MI.
  • Buchman SR; M. Haskell Newman Collegiate Professor of Plastic Surgery, Professor of Surgery, and Professor of Neurosurgery, Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, MI. Electronic address: sbuchman@med.umich.edu.
J Oral Maxillofac Surg ; 72(3): 559-66, 2014 Mar.
Article em En | MEDLINE | ID: mdl-24342580
PURPOSE: Radiation is known to decrease osteocyte count and function, leading to bone weakening. A treatment strategy to mitigate these consequences could have immense therapeutic ramifications. The authors previously reported significantly decreased osteocyte count and mineralization capacity in a rat model of fracture healing after radiotherapy. They hypothesized that amifostine (AMF) would preserve osteocyte number and function in this model. MATERIALS AND METHODS: Thirty-six rats were divided into 3 groups: fracture, radiated fracture, and radiated fracture with AMF. Radiated groups underwent human-equivalent radiotherapy to the mandible before fixator placement and mandibular osteotomy. The AMF group received a subcutaneous injection before each dose of radiotherapy. After 40 days, mandibles were harvested for histologic processing. Quantification of osteocyte count (Oc), empty lacunae (EL), and osteoid ratio (osteoid volume [OV] to tissue volume [TV]) was performed and the results were compared using analysis of variance (P < .05). RESULTS: Radiated fractures showed significantly decreased Oc, increased EL, and a decreased capacity to produce new osteoid at the fracture site as measured with OV/TV compared with nonradiated fractures. In mandibles treated with AMF, these metrics were not statistically different than the control, indicating a preservation of osteocyte number and function. CONCLUSIONS: These results support the hypothesis that AMF preserves osteocyte number and function, thereby preventing the pernicious effects of radiotherapy on the cellular environment of fracture healing. Based on these findings, the authors encourage future investigation of this promising therapy for use in the prevention of pathologic fractures and osteoradionecrosis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteócitos / Protetores contra Radiação / Radioterapia / Consolidação da Fratura / Amifostina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Oral Maxillofac Surg Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osteócitos / Protetores contra Radiação / Radioterapia / Consolidação da Fratura / Amifostina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Oral Maxillofac Surg Ano de publicação: 2014 Tipo de documento: Article