Coupling 40S ribosome recruitment to modification of a cap-binding initiation factor by eIF3 subunit e.
Genes Dev
; 28(8): 835-40, 2014 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-24736843
ABSTRACT
40S ribosomes are loaded onto capped mRNAs via the multisubunit translation initiation factors eIF3 and eIF4F. While eIF4E is the eIF4F cap recognition component, the eIF4G subunit associates with 40S-bound eIF3. How this intricate process is coordinated remains poorly understood. Here, we identify an eIF3 subunit that regulates eIF4F modification and show that eIF3e is required for inducible eIF4E phosphorylation. Significantly, recruitment of the eIF4E kinase Mnk1 (MAPK signal-integrating kinase 1) to eIF4F depended on eIF3e, and eIF3e was sufficient to promote Mnk1-binding to eIF4G. This establishes a mechanism by which 40S ribosome loading imparts a phosphorylation mark on the cap-binding eIF4F complex that regulates selective mRNA translation and is synchronized by a specific eIF3 subunit.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Subunidades Proteicas
/
Proteínas de Ligação ao Cap de RNA
/
Fator de Iniciação 3 em Eucariotos
/
Subunidades Ribossômicas Menores de Eucariotos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Genes Dev
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos