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Comparative analysis of Hsp10 and Hsp90 expression in healthy mucosa and adenocarcinoma of the large bowel.
Rappa, Francesca; Sciume, Carmelo; Lo Bello, Margherita; Bavisotto, Celeste Caruso; Marino Gammazza, Antonella; Barone, Rosario; Campanella, Claudia; David, Sabrina; Carini, Francesco; Zarcone, Federica; Rizzuto, Stefano; Lena, Adriana; Tomasello, Giovanni; Uzzo, Maria Laura; Spatola, Giovanni Francesco; Bonaventura, Giuseppe; Leone, Angelo; Gerbino, Aldo; Cappello, Francesco; Bucchieri, Fabio; Zummo, Giovanni; Farina, Felicia.
Afiliação
  • Rappa F; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy francyrappa@hotmail.com.
  • Sciume C; Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
  • Lo Bello M; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
  • Bavisotto CC; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
  • Marino Gammazza A; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
  • Barone R; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
  • Campanella C; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
  • David S; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
  • Carini F; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
  • Zarcone F; Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
  • Rizzuto S; Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
  • Lena A; Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
  • Tomasello G; Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy Department of Surgical and Oncological Sciences, University of Palermo, Palermo, Italy.
  • Uzzo ML; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
  • Spatola GF; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
  • Bonaventura G; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
  • Leone A; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
  • Gerbino A; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Histology and Embryology, University of Palermo, Palermo, Italy.
  • Cappello F; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
  • Bucchieri F; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
  • Zummo G; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
  • Farina F; Department of Experimental Biomedicine and Clinical Neuroscience, Section of Human Anatomy, University of Palermo, Palermo, Italy.
Anticancer Res ; 34(8): 4153-9, 2014 Aug.
Article em En | MEDLINE | ID: mdl-25075042
ABSTRACT

BACKGROUND:

Heat shock proteins (Hsps) assist other proteins in their folding and drive the degradation of defective proteins. During evolution, these proteins have also acquired other roles. Hsp10 is involved in immunomodulation and tumor progression. Hsp90 stabilizes a range of "client" proteins involved in cell signaling. The present study evaluated the expression levels of Hsp10 and Hsp90 in normal mucosa and adenocarcinoma samples of human large bowel. MATERIALS AND

METHODS:

Samples of normal mucosa and adenocarcinoma were collected and Reverse transcriptase-polymerase chain reaction RT-PCR, western blotting (WB) analyses, as well as immunohistochemistry were performed to evaluate the expression levels of Hsp10 and Hsp90.

RESULTS:

RT-PCR showed a higher gene expression of Hsp10 and Hsp90 in adenocarcinoma samples compared to healthy mucosa. WB results confirmed these findings. Immunohistochemistry revealed higher levels of Hsp10 in adenocarcinoma in both the epithelium and the lamina propria, while Hsp90 expression was higher in the adenocarcinoma samples only in the lamina propria.

CONCLUSION:

Hsp10 and Hsp90 may be involved in large bowel carcinogenesis.
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Base de dados: MEDLINE Assunto principal: Adenocarcinoma / Neoplasias do Colo / Proteínas de Choque Térmico HSP90 / Chaperonina 10 / Mucosa Intestinal Tipo de estudo: Etiology_studies Limite: Humans Idioma: En Revista: Anticancer Res Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Itália
Buscar no Google
Imprimir
XML
PubMed Links

Base de dados: MEDLINE Assunto principal: Adenocarcinoma / Neoplasias do Colo / Proteínas de Choque Térmico HSP90 / Chaperonina 10 / Mucosa Intestinal Tipo de estudo: Etiology_studies Limite: Humans Idioma: En Revista: Anticancer Res Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Itália