Microsatellite markers for Cariniana legalis (Lecythidaceae) and their transferability to C. estrellensis.
Appl Plant Sci
; 1(6)2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-25202554
ABSTRACT
PREMISE OF THE STUDY Microsatellite primers were developed in the neotropical tree species Cariniana legalis (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow. ⢠METHODS AND RESULTS:
We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 C. legalis specimens and 26 C. estrellensis specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in C. legalis and three to 12 in C. estrellensis. For C. legalis, the observed (H o) and expected (H e) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For C. estrellensis, H o and H e ranged from 0 to 0.96 and from 0.14 to 0.91, respectively. â¢CONCLUSIONS:
The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in C. legalis and the related species C. estrellensis.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Appl Plant Sci
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Brasil