Role for endocytosis of a constitutively active GPCR (GPR185) in releasing vertebrate oocyte meiotic arrest.
Dev Biol
; 395(2): 355-66, 2014 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-25220151
ABSTRACT
Vertebrate oocytes are naturally arrested at prophase of meiosis I for sustained periods of time before resuming meiosis in a process called oocyte maturation that prepares the egg for fertilization. Members of the constitutively active GPR3/6/12 family of G-protein coupled receptors represent important mediators of meiotic arrest. In the frog oocyte the GPR3/12 homolog GPRx (renamed GPR185) has been shown to sustain meiotic arrest by increasing intracellular cAMP levels through GαSßγ. Here we show that GPRx is enriched at the cell membrane (~80%), recycles through an endosomal compartment at steady state, and loses its ability to signal once trapped intracellularly. Progesterone-mediated oocyte maturation is associated with significant internalization of both endogenous and overexpressed GPRx. Furthermore, a GPRx mutant that does not internalize in response to progesterone is significantly more efficient than wild-type GPRx at blocking oocyte maturation. Collectively our results argue that internalization of the constitutively active GPRx is important to release oocyte meiotic arrest.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Xenopus laevis
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Proteínas de Xenopus
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Receptores Acoplados a Proteínas G
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Endocitose
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Pontos de Checagem do Ciclo Celular
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Meiose
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Modelos Biológicos
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2014
Tipo de documento:
Article
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