More efficient repair of DNA double-strand breaks in skeletal muscle stem cells compared to their committed progeny.
Stem Cell Res
; 13(3 Pt A): 492-507, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25262445
ABSTRACT
The loss of genome integrity in adult stem cells results in accelerated tissue aging and is possibly cancerogenic. Adult stem cells in different tissues appear to react robustly to DNA damage. We report that adult skeletal stem (satellite) cells do not primarily respond to radiation-induced DNA double-strand breaks (DSBs) via differentiation and exhibit less apoptosis compared to other myogenic cells. Satellite cells repair these DNA lesions more efficiently than their committed progeny. Importantly, non-proliferating satellite cells and post-mitotic nuclei in the fiber exhibit dramatically distinct repair efficiencies. Altogether, reduction of the repair capacity appears to be more a function of differentiation than of the proliferation status of the muscle cell. Notably, satellite cells retain a high efficiency of DSB repair also when isolated from the natural niche. Finally, we show that repair of DSB substrates is not only very efficient but, surprisingly, also very accurate in satellite cells and that accurate repair depends on the key non-homologous end-joining factor DNA-PKcs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Músculo Esquelético
/
Reparo do DNA por Junção de Extremidades
Limite:
Animals
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2014
Tipo de documento:
Article