Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks.
Methods Enzymol
; 546: 251-71, 2014.
Article
em En
| MEDLINE
| ID: mdl-25398344
ABSTRACT
Recurrent chromosomal translocations are found in numerous tumor types, often leading to the formation and expression of fusion genes with oncogenic potential. Creating chromosomal translocations at the relevant endogenous loci, rather than ectopically expressing the fusion genes, opens new possibilities for better characterizing molecular mechanisms driving tumor formation. In this chapter, we describe methods to create cancer translocations in human cells. DSBs or paired nicks generated by either wild-type Cas9 or the Cas9 nickase, respectively, are used to induce translocations at the relevant loci. Using different PCR-based methods, we also explain how to quantify translocation frequency and to analyze breakpoint junctions in the cells of interest. In addition, PCR detection of translocations is used as a very sensitive method to detect off-target effects, which has general utility.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Translocação Genética
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Desoxirribonuclease I
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Quebras de DNA de Cadeia Dupla
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Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
França