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CXCL13 promotes the effect of bone marrow mesenchymal stem cells (MSCs) on tendon-bone healing in rats and in C3HIOT1/2 cells.
Tian, Feng; Ji, Xiang-Lu; Xiao, Wan-An; Wang, Bin; Wang, Fei.
Afiliação
  • Tian F; Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. ftiangogo@163.com.
  • Ji XL; Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Xianglu_Ji@163.com.
  • Xiao WA; Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Wan_anXiao@163.com.
  • Wang B; Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Bin_Wang1011@163.com.
  • Wang F; Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China. Fei_Wang1001@163.com.
Int J Mol Sci ; 16(2): 3178-87, 2015 Jan 30.
Article em En | MEDLINE | ID: mdl-25647417
OBJECTIVES: Mesenchymal stem cells (MSCs) are potential effective therapy for tissue repair and bone regeneration. In present study, the effects of CXC chemokine ligand-13 (CXCL13) were evaluated on tendon-bone healing of rats. METHODS: Tendon bone healing of the rat model was established and biomechanical testing was performed at 2, 4, 8 weeks after surgery. Murine mesenchymal cell line (C3HIOT1/2 cells) was cultured. The expression of miRNA-23a was detected by real-time PCR. The protein expression of ERK1/2, JNK and p38 was detected by western blotting. MiR-23a mimic and inhibitor were used to overexpress or silence the expression of miR-23a. RESULTS: MSCs significantly elevated the levels of ultimate load to failure, stiffness and stress in specimens of rats, the effects of which were enhanced by CXCL13. The expression of miR-23a was down-regulated and the protein of ERK1/2 level was up-regulated by CXCL13 treatment in both in vivo and in vitro experiments. ERK1/2 expression was elevated by overexpression of miR-23a and reduced by miR-23a inhibitor. CONCLUSIONS: These findings revealed that CXCL13 promoted the tendon-bone healing in rats with MSCs treatment, and implied that the activation of ERK1/2 via miR-23a was involved in the process of MSCs treated bone regeneration.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismos dos Tendões / Cicatrização / Quimiocina CXCL13 / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traumatismos dos Tendões / Cicatrização / Quimiocina CXCL13 / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China