Specificity and nonspecificity in RNA-protein interactions.
Nat Rev Mol Cell Biol
; 16(9): 533-44, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26285679
ABSTRACT
To fully understand the regulation of gene expression, it is critical to quantitatively define whether and how RNA-binding proteins (RBPs) discriminate between alternative binding sites in RNAs. Here, we describe new methods that measure protein binding to large numbers of RNA variants, and ways to analyse and interpret data obtained by these approaches, including affinity distributions and free energy landscapes. We discuss how the new methodologies and the associated concepts enable the development of inclusive, quantitative models for RNA-protein interactions that transcend the traditional binary classification of RBPs as either specific or nonspecific.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
/
Proteínas de Ligação a RNA
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Rev Mol Cell Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos