Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.
Anal Chem
; 87(23): 11660-5, 2015 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-26456715
ABSTRACT
We introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). We also developed an additional lateral flow assay for IgE, in which the analyte is sandwiched between immobilized anti-IgE antibodies and aptamer-bearing reporter phage modified with HRP. The limit of detection of this LFA was 0.13 ng/mL IgE, â¼100 times lower than those of previously reported IgE assays.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
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Imunoglobulina E
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Bacteriófago M13
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Proteínas de Ligação às Penicilinas
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Aptâmeros de Peptídeos
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos