Prolactin protects retinal pigment epithelium by inhibiting sirtuin 2-dependent cell death.
EBioMedicine
; 7: 35-49, 2016 May.
Article
em En
| MEDLINE
| ID: mdl-27322457
ABSTRACT
The identification of pathways necessary for retinal pigment epithelium (RPE) function is fundamental to uncover therapies for blindness. Prolactin (PRL) receptors are expressed in the retina, but nothing is known about the role of PRL in RPE. Using the adult RPE 19 (ARPE-19) human cell line and mouse RPE, we identified the presence of PRL receptors and demonstrated that PRL is necessary for RPE cell survival via anti-apoptotic and antioxidant actions. PRL promotes the antioxidant capacity of ARPE-19 cells by reducing glutathione. It also blocks the hydrogen peroxide-induced increase in deacetylase sirtuin 2 (SIRT2) expression, which inhibits the TRPM2-mediated intracellular Ca(2+) rise associated with reduced survival under oxidant conditions. RPE from PRL receptor-null (prlr(-/-)) mice showed increased levels of oxidative stress, Sirt2 expression and apoptosis, effects that were exacerbated in animals with advancing age. These observations identify PRL as a regulator of RPE homeostasis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prolactina
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Envelhecimento
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Canais de Cátion TRPM
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Epitélio Pigmentado da Retina
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Sirtuína 2
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
EBioMedicine
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
México