Nanoparticles size-dependently initiate self-limiting NETosis-driven inflammation.
Proc Natl Acad Sci U S A
; 113(40): E5856-E5865, 2016 10 04.
Article
em En
| MEDLINE
| ID: mdl-27647892
ABSTRACT
The critical size for strong interaction of hydrophobic particles with phospholipid bilayers has been predicted to be 10 nm. Because of the wide spreading of nonpolar nanoparticles (NPs) in the environment, we aimed to reveal the ability of living organisms to entrap NPs via formation of neutrophil extracellular traps (NETs). Upon interaction with various cell types and tissues, 10- to 40-nm-sized NPs induce fast (<20 min) damage of plasma membranes and instability of the lysosomal compartment, leading to the immediate formation of NETs. In contrast, particles sized 100-1,000 nm behaved rather inertly. Resulting NET formation (NETosis) was accompanied by an inflammatory reaction intrinsically endowed with its own resolution, demonstrated in lungs and air pouches of mice. Persistence of small NPs in joints caused unremitting arthritis and bone remodeling. Small NPs coinjected with antigen exerted adjuvant-like activity. This report demonstrates a cellular mechanism that explains how small NPs activate the NETosis pathway and drive their entrapping and resolution of the initial inflammatory response.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tamanho da Partícula
/
Nanopartículas
/
Armadilhas Extracelulares
/
Inflamação
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha