Your browser doesn't support javascript.
loading
Identification of Donor Origin and Condition of Transplanted Islets In Situ in the Liver of a Type 1 Diabetic Recipient.
Cell Transplant ; 26(1): 1-9, 2017 01 24.
Article em En | MEDLINE | ID: mdl-27729094
Transplantation of islet allografts into type 1 diabetic recipients usually requires multiple pancreas donors to achieve insulin independence. This adds to the challenges of immunological monitoring of islet transplantation currently relying on surrogate immune markers in peripheral blood. We investigated donor origin and infiltration of islets transplanted in the liver of a T1D patient who died of hemorrhagic stroke 4 months after successful transplantation with two intraportal islet grafts combining six donors. Immunohistological staining for donor HLA using a unique panel of human monoclonal HLA-specific alloantibodies was performed on liver cryosections after validation on cryopreserved kidney, liver, and pancreas and compared with auto- and alloreactive T-cell immunity in peripheral blood. HLA-specific staining intensity and signal-to-noise ratio varied between tissues from very strong on kidney glomeruli, less in liver, kidney tubuli, and endocrine pancreas to least in exocrine pancreas, complicating the staining of inflamed islets in an HLA-disparate liver. Nonetheless, five islets from different liver lobes could be attributed to donors 1, 2, and 5 by staining patterns with multiple HLA types. All islets showed infiltration with CD8+ cytotoxic T cells that was mirrored by progressive alloreactive responses in peripheral blood mononuclear cells (PBMCs) to donors 1, 2, and 5 after transplantation. Stably low rates of peripheral islet autoreactive T-cell responses after islet infusion fit with a complete HLA mismatch between grafts and recipient and exclude the possibility that the islet-infiltrating CD8 T cells were autoreactive. HLA-specific immunohistochemistry can identify donor origin in situ and differentiate graft dysfunction and immunological destruction.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doadores de Tecidos / Transplante das Ilhotas Pancreáticas / Diabetes Mellitus Tipo 1 Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Middle aged Idioma: En Revista: Cell Transplant Assunto da revista: TRANSPLANTE Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doadores de Tecidos / Transplante das Ilhotas Pancreáticas / Diabetes Mellitus Tipo 1 Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Middle aged Idioma: En Revista: Cell Transplant Assunto da revista: TRANSPLANTE Ano de publicação: 2017 Tipo de documento: Article