A Fluorophore Fusion Construct of Human Profilin I with Non-Compromised Poly(L-Proline) Binding Capacity Suitable for Imaging.

Nejedla, Michaela; Li, Zhilun; Masser, Anna E; Biancospino, Matteo; Spiess, Matthias; Mackowiak, Sebastian D; Friedländer, Marc R; Karlsson, Roger

Nejedla, Michaela; Li, Zhilun; Masser, Anna E; Biancospino, Matteo; Spiess, Matthias; Mackowiak, Sebastian D; Friedländer, Marc R; Karlsson, Roger.

Afiliação

Nejedla M; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.
Li Z; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.
Masser AE; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.
Biancospino M; Fondazione Istituto FIRC di Oncologia Molecolare (IFOM), IT-20139 Milan, Italy.
Spiess M; Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83 Huddinge, Sweden.
Mackowiak SD; Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
Friedländer MR; Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
Karlsson R; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address: roger.karlsson@su.se.

J Mol Biol ; 429(7): 964-976, 2017 04 07.

Article em En | MEDLINE | ID: mdl-28077285

Assuntos

Proteínas de Bactérias/genética; Proteínas de Bactérias/metabolismo; Proteínas Luminescentes/genética; Proteínas Luminescentes/metabolismo; Peptídeos/metabolismo; Profilinas/genética; Profilinas/metabolismo; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes de Fusão/metabolismo; Actinas/metabolismo; Humanos; Fosfatidilinositóis/metabolismo; Ligação Proteica

Palavras-chave

actin binding; intramolecular citrine fusion; poly-proline and phosphatidylinositol lipid interaction

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos / Proteínas de Bactérias / Proteínas Recombinantes de Fusão / Profilinas / Proteínas Luminescentes Limite: Humans Idioma: En Revista: J Mol Biol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Suécia

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