Microbubble-Based Sonothrombolysis Using a Planar Rectangular Ultrasonic Transducer.
J Stroke Cerebrovasc Dis
; 26(6): 1287-1296, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28236599
ABSTRACT
BACKGROUND:
The aim of the proposed study was to evaluate in an in vitro flow model the ability of small planar rectangular (2 × 10 mm2) ultrasonic transducer to enhance thrombolysis induced by the thrombolytic agent tenecteplase (TNK-tPA).METHODS:
To provide a more realistic clinical environment of stroke, the study was conducted under realistic flow conditions and TNK-tPA concentrations. Fully retracted porcine blood clots were used to determine the thrombolytic efficacy of ultrasound (US) waves as an adjunct to TNK-tPA or in combination with microbubbles (MBs). Two ultrasonic flat rectangular transducers were used in the experiments, operating at 3.7 and 5.2 MHz respectively. A pulsed US protocol that maintained temperature elevation at the target of 1°C was applied. Thrombolysis efficacy was measured in milligrams of mass clot removed.RESULTS:
The effect of experimental parameters, such as power, frequency, and MBs administration, on thrombolysis efficacy was explored.CONCLUSIONS:
The results revealed that thrombolysis efficacy decreases at higher frequency, and therefore, the possibility of using lower frequency to improve efficacy should be further investigated. Additionally, study findings demonstrated that the combination of 3.7 MHz with MBs as an adjunct to TNK-tPA strongly enhanced thrombolysis efficacy, because with 30 minutes of treatment, 700 mg of clot was removed through nonthermal mechanisms. As a final point, this study has shown that MBs dose influences thrombolysis enhancement, because higher thrombolytic efficacy was observed with higher doses of MBs.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Hexafluoreto de Enxofre
/
Transdutores
/
Terapia por Ultrassom
/
Coagulação Sanguínea
/
Terapia Trombolítica
/
Ativador de Plasminogênio Tecidual
/
Meios de Contraste
/
Acidente Vascular Cerebral
/
Microbolhas
Tipo de estudo:
Evaluation_studies
/
Guideline
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Stroke Cerebrovasc Dis
Assunto da revista:
ANGIOLOGIA
/
CEREBRO
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido