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Medium pH in submerged cultivation modulates differences in the intracellular protein profile of Fusarium oxysporum.
da Rosa-Garzon, Nathália Gonsales; Laure, Hélen Julie; Souza-Motta, Cristina Maria de; Rosa, José César; Cabral, Hamilton.
Afiliação
  • da Rosa-Garzon NG; a School of Pharmaceutical Sciences of Ribeirão Preto, Department of Pharmaceutical Sciences , University of São Paulo , Ribeirão Preto , SP , Brazil.
  • Laure HJ; b School of Medicine of Ribeirão Preto, Department of Molecular and Cellular Biology and Pathogenic Bioagents , University of São Paulo , Ribeirão Preto , SP , Brazil.
  • Souza-Motta CM; c Department of Mycology , Federal University of Pernambuco , Recife , PE , Brazil.
  • Rosa JC; b School of Medicine of Ribeirão Preto, Department of Molecular and Cellular Biology and Pathogenic Bioagents , University of São Paulo , Ribeirão Preto , SP , Brazil.
  • Cabral H; a School of Pharmaceutical Sciences of Ribeirão Preto, Department of Pharmaceutical Sciences , University of São Paulo , Ribeirão Preto , SP , Brazil.
Prep Biochem Biotechnol ; 47(7): 664-672, 2017 Aug 09.
Article em En | MEDLINE | ID: mdl-28281888
ABSTRACT
Fusarium oxysporum is a filamentous fungus that damages a wide range of plants and thus causes severe crop losses. In fungal pathogens, the genes and proteins involved in virulence are known to be controlled by environmental pH. Here, we report the influence of culture-medium pH (5, 6, 7, and 8) on the production of degradative enzymes involved in the pathogenesis of F. oxysporum URM 7401 and on the 2D-electrophoresis profile of intracellular proteins in this fungus. F. oxysporum URM 7401 was grown in acidic, neutral, and alkaline culture media in a submerged bioprocess. After 96 hr, the crude extract was processed to enzyme activity assays, while the intracellular proteins were obtained from mycelium and analyzed using 2D electrophoresis and mass spectrometry. We note that the diversity of secreted enzymes was changed quantitatively in different culture-medium pH. Also, the highest accumulated biomass and the intracellular protein profile of F. oxysporum URM 7401 indicate an increase in metabolism in neutral-alkaline conditions. The differential profiles of secreted enzymes and intracellular proteins under the evaluated conditions indicate that the global protein content in F. oxysporum URM 7401 is modulated by extracellular pH.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Proteínas Fúngicas / Meios de Cultura / Fusarium Idioma: En Revista: Prep Biochem Biotechnol Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Proteínas Fúngicas / Meios de Cultura / Fusarium Idioma: En Revista: Prep Biochem Biotechnol Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil