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Age and sex differences in human skeletal muscle fibrosis markers and transforming growth factor-ß signaling.
Parker, Lewan; Caldow, Marissa K; Watts, Rani; Levinger, Pazit; Cameron-Smith, David; Levinger, Itamar.
Afiliação
  • Parker L; Institute of Sport, Exercise and Active Living (ISEAL), Victoria University, PO Box 14428, Melbourne, VIC, 8001, Australia.
  • Caldow MK; Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Burwood, VIC, Australia.
  • Watts R; Basic and Clinical Myology Laboratory, Department of Physiology, University of Melbourne, Melbourne, Australia.
  • Levinger P; Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Burwood, VIC, Australia.
  • Cameron-Smith D; Centre for Exercise and Nutrition, The Mary MacKillop Institute for Health Research, Australian Catholic University, Fitzroy, VIC, Australia.
  • Levinger I; Institute of Sport, Exercise and Active Living (ISEAL), Victoria University, PO Box 14428, Melbourne, VIC, 8001, Australia.
Eur J Appl Physiol ; 117(7): 1463-1472, 2017 Jul.
Article em En | MEDLINE | ID: mdl-28493029
PURPOSE: The aim of the study was to determine whether higher fibrosis markers in skeletal muscle of older adults are accompanied by increased expression of components of the canonical TGF-ß signal transduction pathway. METHODS: Fourteen healthy young (21-35 years; 9 males and 5 females) and seventeen older (55-75 years; 9 males and 8 females) participants underwent vastus lateralis biopsies to determine intramuscular mRNA and protein expression of fibrogenic markers and TGF-ß signaling molecules related to TGF-ß1 and myostatin. RESULTS: Expression of mRNA encoding the pro-fibrotic factors; axin 2, collagen III, ß-catenin and fibronectin, were all significantly higher (all p < 0.05) in the older participants (350, 170, 298, and 641%, respectively). Furthermore, axin 2 and ß-catenin mRNA were significantly higher in older females than older males (p < 0.05). Gene expression of ActRIIB, myostatin, and TGF-ß1 were higher in older adults compared to younger adults (all p < 0.05). There was, however, no difference in the total protein content of myostatin, myoD or myogenin (all p > 0.05), whereas Smad3 protein phosphorylation was 48% lower (p < 0.05) in muscle from older adults. CONCLUSIONS: Increased abundance of mRNA of fibrotic markers was observed in muscle from older adults and was partly accompanied by altered abundance of pro-fibrotic ligands in a sex specific manner.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Envelhecimento / Transdução de Sinais / Fator de Crescimento Transformador beta / Fibronectinas / Músculo Esquelético / Colágeno Tipo III Limite: Adult / Aged / Female / Humans / Male Idioma: En Revista: Eur J Appl Physiol Assunto da revista: FISIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Envelhecimento / Transdução de Sinais / Fator de Crescimento Transformador beta / Fibronectinas / Músculo Esquelético / Colágeno Tipo III Limite: Adult / Aged / Female / Humans / Male Idioma: En Revista: Eur J Appl Physiol Assunto da revista: FISIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália