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Characterization of mobile genetic elements carrying VIM-1 and KPC-2 carbapenemases in Citrobacter freundii isolates in Madrid.
Villa, Jennifer; Arana, David M; Viedma, Esther; Perez-Montarelo, Dafne; Chaves, Fernando.
Afiliação
  • Villa J; Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain. Electronic address: jennifer.villa@salud.madrid.org.
  • Arana DM; Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Viedma E; Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Perez-Montarelo D; Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Chaves F; Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain.
Int J Med Microbiol ; 307(6): 340-345, 2017 Sep.
Article em En | MEDLINE | ID: mdl-28711315
Carbapenemase producing Citrobacter freundii (CPCF) infections are still uncommon in European countries. Here we report a molecular study conducted in a tertiary care facility in southern Madrid, Spain, from 2009 to 2014 to investigate the epidemiology of CPCF. The blaIMP-1,blaIMP-2,blaKPC,blaNDM,blaOXA-48,blaVIM-1 and blaVIM-2 genes were screened by PCR. Molecular typing was carried out by Pulsed-field gel electrophoresis analysis (PFGE) and multilocus sequence typing (MLST). Whole genome sequencing (WGS) was performed to characterize the resistome and the mobile genetic elements associated with the carbapenems resistance of CPCF. A total of 11/521 (2.1%) isolates had reduced susceptibility to carbapenems. PCR amplification revealed the presence of blaVIM-1 in 10 isolates and blaKPC-2 in 2 isolates. One C. freundii isolate co-harbored blaVIM-1 and blaKPC-2 genes. PFGE and MLST assigned 10 different clonal, 4 previously reported (ST11, ST18, ST22 and ST64) and 6 new STs (ST89, ST90, ST91, ST92, ST92 and ST94). The blaVIM-1 gene was part of In624 (intI1-blaVIM-1-aacA4-dfrB1-aadA1-catB2-qacEΔ1/sul1). In 3 of these isolates, plasmid-mediated quinolone resistance genes (qnrA1 and qnrB4) were present in its downstream region, taking part of a complex class 1 integron ([In624:ISCR1:qnrB4-blaDHA-1] and [In624:ISCR1:qnrA1]). On the other hand, the blaKPC-2 gene was associated with a Tn3-based transposon. The dissemination of the blaVIM-1 gene among various clones suggests a successful horizontal transfer of integron carrying elements that play a dominant role in the development of multidrug resistance in Enterobacteriaceae.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Beta-Lactamases / Citrobacter freundii / Sequências Repetitivas Dispersas / Farmacorresistência Bacteriana Múltipla / Infecções por Enterobacteriaceae Limite: Animals / Humans País/Região como assunto: Europa Idioma: En Revista: Int J Med Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Beta-Lactamases / Citrobacter freundii / Sequências Repetitivas Dispersas / Farmacorresistência Bacteriana Múltipla / Infecções por Enterobacteriaceae Limite: Animals / Humans País/Região como assunto: Europa Idioma: En Revista: Int J Med Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article