Reconstructing human pancreatic differentiation by mapping specific cell populations during development.
Elife
; 62017 07 21.
Article
em En
| MEDLINE
| ID: mdl-28731406
ABSTRACT
Information remains scarce on human development compared to animal models. Here, we reconstructed human fetal pancreatic differentiation using cell surface markers. We demonstrate that at 7weeks of development, the glycoprotein 2 (GP2) marks a multipotent cell population that will differentiate into the acinar, ductal or endocrine lineages. Development towards the acinar lineage is paralleled by an increase in GP2 expression. Conversely, a subset of the GP2+ population undergoes endocrine differentiation by down-regulating GP2 and CD142 and turning on NEUROG3, a marker of endocrine differentiation. Endocrine maturation progresses by up-regulating SUSD2 and lowering ECAD levels. Finally, in vitro differentiation of pancreatic endocrine cells derived from human pluripotent stem cells mimics key in vivo events. Our work paves the way to extend our understanding of the origin of mature human pancreatic cell types and how such lineage decisions are regulated.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
Biomarcadores
/
Diferenciação Celular
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Regulação da Expressão Gênica no Desenvolvimento
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Linhagem da Célula
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Feto
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Elife
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
França